Biozen Sample Preparation Products
MagBeads
Streptavidin coated paramagnetic particle, ideal for isolating proteins and peptides in less time with a high response. With a unique magnetic bead particle and rigorous specifications, Biozen MagBeads are created to ensure uniform beads that provide consistent and reliable results. Pairs with: Biozen XB-Peptide and Peptide LC ColumnsN-Glycan Clean-Up SPE
Solid phase extraction (SPE) HILIC stationary phase that excels at retention and recovery labeled released N-glycans! Microelution format allows for streamlined processing and clean-up of small sample volumes.
Peptide MagBeads
Streptavidin Coated
Uniform magnetic particles result in faster and reliable purification, clean-up, and isolation of proteins and peptide molecules.
MagBead Activation
Immunocapture
Glycan: N-Glycan Clean-Up
Streptavidin Coated
Uniform magnetic particles result in faster and reliable purification, clean-up, and isolation of proteins and peptide molecules. STEP 1 |
 STEP 2 |
 STEP 3 |
 STEP 4 |
 STEP 5 |
| Start with Intact Glycoprotein | Deglycosylation Released N-Glycans by PNGase F in buffer solution |
Labeling Glycans 2-AA or 2-AB for accurate and sensitive glycan detection |
Clean-Up Removal of labeling reagent, salts, or surfactants |
Analysis |
|
96-Well Plate:
|
|
Part No.:
8M-S009-NGA
|
|
Condition:
200 µL Water
|
|
Equilibrate:
200 µL Water/Acetonitrile (15:85)
|
|
Load:
Labeled Sample (up to 400 µL of labeled sample)
|
|
Wash:
2x 600 µL Formic acid/Water/Acetonitrile (1:9:90)
|
|
Elute:
3x 30 µL 200 mM Ammonium acetate in Acetonitrile/Water (5:95) of labeled sample)
|
|
Dilute:
100 µL Dimethylformamide and then 210 µL of Acetonitrile
|
Pair with a Biozen LC Column
| Intact | |
|---|---|
| Biozen Intact XB-C8 3.6 µm |
Biozen WidePore C4 2.6 µm |
 Large pore core-shell particle for fast intact and subunit biologic entry. C8 provides highly useful moderate hydrophobic selectivity. |
 Core-shell particle with butyl stationary phase and optimal wide pore size distribution for better resolution of large biologics, including monoclonal antibodies and subunit analysis. |
| Size Exclusion (SEC) | |
|---|---|
| Biozen SEC-2 1.8 µm |
Biozen SEC-3 1.8 µm |
 Large pore core-shell particle for fast intact and subunit biologic entry. C8 provides highly useful moderate hydrophobic selectivity. |
 Extremely inert, high density fully porous particle with high efficiency and high molecular weight (HMW) separation range of 10 k – 700 kDa. |
| Peptide | |
|---|---|
| Biozen Peptide XB-C18 1.7 µm and 2.6 µm |
Biozen Peptide PS-C18 1.6 µm and 3 µm |
 Overall retention of both acidic and basic peptides through C18 stationary phase with di-isobutyl side chains. |
 Excellent retention by combined positively charged surface ligand and C18 ligand. |
| Ion-Exchange | Glycan |
|---|---|
| Biozen WCX 6 µm |
Biozen Glycan 2.6 µm |
 Monosized particles grafted with linear polycarboxylate chains to envelop and separate proteins from acidic/basic variants |
Provides optimal combination of high efficiency and selectivity for released glycans. |
| Oligonucleotides |
|---|
| bioZen Oligo 1.7 µm and 2.6 µm |
 Organo-silica core-shell particle bonded with a C18 stationary phase offers high selectivity for even minute oligo differences alongside high and low pH robustness. |
