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Product Information
Phase Detail
BioZen Peptide-XB-C18
BioZen Peptide-XB-C18
BioZen Peptide-XB-C18
Digested mAbs or ADCs typically include a large body of compounds which are crucial to understanding post translation modifications. So we designed our Biozenâ„¢ Peptide columns to offer highly useful and unique retention profiles. Biozen Peptide XB-C18 allows for fast and effective elution windows by utilizing core-shell particles to gain sharper peaks, better peak capacities, and overall higher sensitivity.
Biozen Peptide columns provide:
- Unique retention profiles
- Greater peak coverage
- High efficiency on both HPLC and UHPLC
Separation Mode Reversed Phase |
Particle Size 1.7, 2.6, 5 |
Recommended Use Overall retention of both acidic and basic peptides for both peptide mapping and peptide quantitation applications |
Inside bioZen BioTi Hardware
Conditions for both columns:
Column:
bioZen 1.8 μm SEC-3 |
Dimension:
150 x 4.6 mm |
Mobile Phase:
50 mM Dipotassium Phosphate + 100 mM Sodium Sulfate, pH 5.0 |
Flow Rate:
0.3 mL/min |
Temperature:
Ambient |
Detection:
UV @ 280 nm |
Sample:
1. γ-Globulin, 5 mg/mL2. Ovalbumin, 1 mg/mL |
Peptide Mapping: Reversed Phase LC
Cetuximab Peptide Map
Anti Rituximab Fab Peptide Map
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Columns:
 bioZen 1.6 µm Peptide PS-C18 bioZen 2.6 µm Peptide XB-C18 |
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Dimension:
150 x 2.1 mm
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Part No.:
00F-4770-AN
00F-4768-AN |
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Mobile Phase:
A: 0.1 % TFA in Water
B: 0.1 % TFA in Acetonitrile |
Gradient:
Time (min)
0
5 60 65 70 75 78 | % B
1
1 50 50 95 95 95 |
Flow Rate:
bioZen 1.6 µm Peptide PS-C18: 0.3 mL/min bioZen 2.6 µm Peptide XB-C18: 0.6 mL/min |
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Temperature:
40 °C |
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Detection:
UV @ 214 nm |
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Conditions for all columns:
Columns:
bioZen 2.6 μm Peptide XB-C18
bioZen 1.6 m Peptide PS-C18 |
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Dimension:
150 x 2.1 mm
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Part No.:
00F-4768-AN 00F-4770-AN |
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Mobile Phase:
A: 0.1% Formic Acid in Water B: 0.1% Formic Acid in Acetonitrile |
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Gradient:
Time (min)
0
545 | % B
33 48 |
Flow Rate:
0.5 mL/min |
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Temperature:
50 °C |
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Detection:
MS/MS |
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Samples:
1. As Noted |
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TICs for Infliximab, XB-C18 vs. PS-C18
LC-UV conditions
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Column:
bioZen 2.6 µm Peptide XB-C18
bioZen 1.6 µm Peptide PS-C18 |
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Part No.:
00F-4768-AN (XB-C18)
00F-4770-AN (PS-C18) |
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Mobile Phase:
A. 0.1 % Formic Acid in Water
B. 0.1 % Formic Acid in Acetonitrile |
Gradient:
1-50 %  B in 50 minutes |
Flow Rate:
0.3 mL/min |
Detection:
SCIEX® X500B Q-TOF |
Sample:
Infliximab Digest, 1 µg |
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