Biozen WCX was constructed with linear polycarboxylate chains grafted to monosized non-porous polymeric particles, to sinuously envelop and separate proteins from acidic and basic variants. With a highly tuned and controlled manufacturing process, Biozen WCX affords scientists a way to reproducibly characterize heterogeneity with an added advantage of excellent recovery using bioinert titanium column hardware.
Monosized particles grafted with linear polycarboxylate chains to envelop and separate proteins from acidic/basic variants
Our specially crafted polymeric particle is produced with outstanding precision so that you can be confident in increased performance and minimized variation between lots.
Comparative separations may not be representative of all applications.
Columns:
bioZen 6 µm WCX
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Dimension:
250 x 4.6 mm
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Part No.:
00G-4777-EO
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Mobile Phase:
A: CX-1 Gradient Buffer A (pH 5.6)
B: CX-1 Gradient Buffer B (pH 10.2) |
Gradient:
0-100 % B in 20 minutes
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Flow Rate:
1 mL/min
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Temperature:
30 °C
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Detection:
UV @ 280 nm
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Sample:
Trastuzumab
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Note:
CX-1 Gradient Buffers available from Thermo
Fisher Scientificâ„¢ |
Column:
bioZen 6 μm WCX
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Dimension:
250 x 4.6 mm
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Part No.:
00G-4777-E0
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Mobile Phase:
A: 20 mM MES (pH 5.6) B: 20 mM MES + 300 mM NaCl (pH 5.6) |
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Gradient:
Time (min)
0
1 31 31.1 34 35 | % B
15 15 45 100 100 15 |
Flow Rate:
1 mL/min |
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Temperature:
30 °C |
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Detection:
UV @ 280 nm |
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Sample:
Trastuzumab |
Column:
bioZen 6 μm WCX
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Dimension:
250 x 4.6 mm
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Part No.:
00G-4777-E0
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Mobile Phase:
A: CX -1 (pH 5.6) pH Gradient Buffer* B: CX -1 (pH 10.2) pH Gradient Buffer* |
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Gradient:
Time (min)
0
1 21 23 24 | % B
0 0 100 100 0 |
Flow Rate:
1 mL/min |
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Temperature:
30 °C |
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Detection:
UV @ 280 nm |
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Sample:
Trastuzumab |
* From Thermo Fisher Scientific® Inc.
Whether using a pH gradient or salt gradient, the bioZen 6μm WCX consistently outperforms ProPac™ WCX-10 columns in charge variant analysis.
Comparative separations may not be representative of all applications.
Columns:
bioZen 6 µm WCX
ProPac 10 µm WCX-10 |
Dimension:
250 x 4.6 mm (bioZen)
250 x 4 mm (ProPac) |
Mobile Phase:
A: CX-1 Gradient Buffer A (pH 5.6)
B: CX-1 Gradient Buffer B (pH 10.2) |
Gradient:
0-100 % B in 20 minutes
|
Flow Rate:
1.0 mL/min (bioZen)
0.8 mL/min (ProPac) |
Temperature:
30 °C
|
Detection:
UV @ 280 nm
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Sample:
As indicated
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Note:
CX-1 Gradient Buffers available from Thermo Fisher Scientificâ„¢
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Columns:
bioZen 6 μm WCX
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Dimension:
50 x 2.1 mm
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Part No.:
00B 4777 AN
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Mobile Phase:
A: 20 mM Ammonium Acetate, pH 5.2
B: 5 mM Ammonium Acetate, pH 10.2 |
Gradient:
20-50 % in 5 min
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Flow Rate:
0.5 mL/min
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Temperature:
30 °C
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Detection:
SCIEX® X500B Q-TOF
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Sample:
Trastuzumab, 50 µg
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Columns:
bioZen 6 μm WCX
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Dimension:
50 x 2.1 mm
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Part No.:
00B 4777 AN
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Mobile Phase:
A: 20 mM Ammonium Acetate, pH 5.2
B: 5 mM Ammonium Acetate, pH 10.2 |
Gradient:
20-50 % in 5 min
|
Flow Rate:
0.5 mL/min
|
Temperature:
30 °C
|
Detection:
SCIEX® X500B Q-TOF
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Sample:
Trastuzumab, 50 µg
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