Biozen Oligo
Advanced Oligonucleotide Analysis
Biozen Oligo is an organo-silica, core-shell particle bonded with a C18 stationary phase. This column offers a unique combination of Core-Shell performance gains such as speed, sensitivity and resolution plus the high pH ruggedness necessary for oligonucleotide separations. Additionally, Biozen Oligo is packaged in BioTiâ„¢, a novel bio-inert hardware that serves to alleviate the sample loss and adsorption issues.
Increased Recovery and Reproducibility with NEW Biozen Oligo
- BioTiâ„¢ Hardware Reduces Sample Loss and Adsorption
- Robustness at High pH and Temperature
- Core-Shell Advantage for High Efficiency
Patented Technology and Advanced Core-Shell Particle Chemistry
Biozen Oligo utilizes organo-silica core-shell technology to:​
- Minimize band broadening associated with diffusion and oligonucleotide mass transfer kinetics​
- Increase efficiency to produce narrower peaks, ideal for resolving closely eluting impurities​
- Patented grafting process ensures high pH and temperature resistance from ethylene cross-linking.​
BioTi versus Traditional Stainless Steel Hardware
LC-MS Analysis of Nusinersen
Oligos can chelate to trace heavy metals in stainless steel column hardware, leading to poor recovery, inconsistent chromatography and problematic carryover. The Biozen Oligo bio-inert hardware provides greater sensitivity as well as improved recovery, demonstrating this column’s optimal utility for oligonucleotide characterization and quantitation.
BioTi vs Stainless Steel Column Hardware​
Biozen Oligo's bioinert hardware enhances recovery, sensitivity, and chromatographic reproducibility by mitigating non-specific adsorption. Stainless steel equivalents fails to prevent this resulting in poor recovery, peak tailing, and residual carryover.​
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