search
Documents
Fast, Simple Urine Drug Extraction with In-well Room Temperature Hydrolysis and Enzyme Removal to Extend LC Column Life
In this technical note, we evaluate the hydrolysis efficiency of IMCSzyme RT by 1) hydrolyzing 4 human urine samples at a range of enzyme amounts, and 2) hydrolyzing 96 human urine samples at a fixed amount of enzyme. We also evaluated how sample treatment using β-Gone™ Plus β-glucuronidase removal plates can improve column life and peak quality of analytes compared to Dilute-and-Shoot treatment methods, using a Kinetex™ 2.6 µm Biphenyl LC column.
Fast, Simple Urine Drug Extraction with In-well Room Temperature Hydrolysis and Enzyme Removal to Extend LC Column Life
In this technical note, we evaluate the hydrolysis efficiency of IMCSzyme RT by 1) hydrolyzing 4 human urine samples at a range of enzyme amounts, and 2) hydrolyzing 96 human urine samples at a fixed amount of enzyme. We also evaluated how sample treatment using β-Gone™ Plus β-glucuronidase removal plates can improve column life and peak quality of analytes compared to Dilute-and-Shoot treatment methods, using a Kinetex™ 2.6 µm Biphenyl LC column.
Document Type:
Technical Notes
Target Industries:
Techniques:
Separation Modes:
Beta-Glucuronidase Removal, Reversed Phase, Solid Phase Extraction
Phases:
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide.
