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Fast, Simple Urine Drug Extraction with In-well Room Temperature Hydrolysis and Enzyme Removal to Extend LC Column Life
In this technical note, we evaluate the hydrolysis efficiency of IMCSzyme RT by 1) hydrolyzing 4 human urine samples at a range of enzyme amounts, and 2) hydrolyzing 96 human urine samples at a fixed amount of enzyme. We also evaluated how sample treatment using β-Gone™ Plus β-glucuronidase removal plates can improve column life and peak quality of analytes compared to Dilute-and-Shoot treatment methods, using a Kinetex™ 2.6 µm Biphenyl LC column.
Fast, Simple Urine Drug Extraction with In-well Room Temperature Hydrolysis and Enzyme Removal to Extend LC Column Life
In this technical note, we evaluate the hydrolysis efficiency of IMCSzyme RT by 1) hydrolyzing 4 human urine samples at a range of enzyme amounts, and 2) hydrolyzing 96 human urine samples at a fixed amount of enzyme. We also evaluated how sample treatment using β-Gone™ Plus β-glucuronidase removal plates can improve column life and peak quality of analytes compared to Dilute-and-Shoot treatment methods, using a Kinetex™ 2.6 µm Biphenyl LC column.
Document Type:
Technical Notes
Target Industries:
Separation Modes:
Beta-Glucuronidase Removal, Reversed Phase, Solid Phase Extraction
Phases:
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Fast, Simple Urine Drug Extraction with In-well Room Temperature Hydrolysis and Enzyme Removal to Extend LC Column Life
In this technical note, we evaluate the hydrolysis efficiency of IMCSzyme RT by 1) hydrolyzing 4 human urine samples at a range of enzyme amounts, and 2) hydrolyzing 96 human urine samples at a fixed amount of enzyme. We also evaluated how sample treatment using β-Gone™ Plus β-glucuronidase removal plates can improve column life and peak quality of analytes compared to Dilute-and-Shoot treatment methods, using a Kinetex™ 2.6 µm Biphenyl LC column.
| Target Industries: | Clinical Research, Forensic Toxicology |
| Techniques: | Beta-Glucuronidase, HPLC, HPLC Reversed Phase, Sample Preparation, Solid Phase Extraction |
| Brands: | Beta-Gone, Kinetex |
| Separation Modes: | Beta-Glucuronidase Removal, Reversed Phase, Solid Phase Extraction |
| Phases: | Kinetex Biphenyl |
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide