Claricep Flash Columns
Seamlessly Upgrade to Claricep Flash Chromatography Columns
Claricep Flash Columns were developed by Agela Technologies to improve performance and decrease undesirable silica surface activity compared to traditional flash columns.
The ordinary, commercial grade silica used in most Flash columns contains impurities (such as heavy metals) which create areas of high surface activity that can distort the interaction of adsorbed solutes with the bulk silica surface. This is manifested in peak shifting, broadening and tailing, particularly for basic and acidic compounds, with Claricep Flash columns have less surface activity than ordinary silica columns. They demonstrate significantly improved chromatographic performance. Available in two flash medias (Irregular and Spherical) with multiple chemistries optimized for resolution and high loading capacity.
Claricep Column
- The deactivated silica surface promotes compound stability
- Excellent peak shape and performance for both acidic and basic compounds
- High quality and reproducibility
- Wide range of selectivities
- High pressure tolerance
Flash Chemistries/Media
Claricep CM utilizes silica that is further purified with a proprietary acid washing process which results in even lower surface activity to result in an improved peak shape and resolution.
- Consistent High Perofrmance
- Wide Range of Selectivities
- High Pressure Tolerance
- Excellent Availability
- High Quality
Comparison of Peak Shape with CM Deactivated vs. Unmodified Silica
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Dimension
4.6 x 150 mm
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Mobile Phase
Dichloromethane/Methanol (98:2)
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Flow Rate
1.8 mL/min |
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Injection Volume
5 μL |
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Temperature
30 °C |
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Detector:
1.8 mL/min |
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UV @ 254 nm
1.8 mL/min |
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Sample
Catechol 100 μg/mL |
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HPLC TEST
Unmodified and Deactivated Silica were packed into individual
stainless steel columns (4.6 x 150 mm) and then evaluated on a
HPLC System |
Irregular Silica Comparison
Ultra pure packing which results in much less abnormal surface activity. This produces chromatograms with much sharper peaks
Aniline Peak Symmetry and Retention Test
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Column
Claricep Irregular Silica CS (40-60 μm, 60 Å, 40 g)
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Brand I
Flash Irregular Silica (40 g)
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Mobile Phase
Dichloromethane/Methanol (99:1)
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Flow Rate
20 mL/min |
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Detector
UV @ 254 nm |
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Temperature
Ambient |
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Retention Time
CLARICEP CS: 4.090 minBrand I: 4.373 min |
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Sample
Aniline |
Comparison of Claricep CS vs. Popular Brand I
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Column
Claricep Irregular Silica CS
(40-60 μm, 60 Å, 40 g) |
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Brand I
Flash Irregular Silica Column (40 g)
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Mobile Phase
Hexane/Ethylacetate (gradient)
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Detector
UV @ 254 nm |
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Temperature
Ambient |
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Retention Time
CLARICEP CS: 4.090 minBrand I: 4.373 min |
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Sample
Phenyl acetone,4-aminobenzoic acid |
Spherical Silica Comparison
Regular, similar-sized and high quality particles can be packed in a column much more uniformly which reduces eddy flow currents and lateral diffusion. The result is greater peak symmetry and baseline resolution than is possible with irregular silica particles.
Claricep Spherical Silica 20-30 µm vs. Claricep Spherical Silica 20 µm
Column A
Claricep Spherical Silica (20-35 μm, 100 Å, 12 g,
2 columns in tandem)
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Column B
Claricep Spherical Silica (20 μm, 100 Å, 12 g,
2 columns in tandem
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Mobile Phase:
A: Hexane B: Ethanol |
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Gradient:
Time (min)
0
20 | % B
5 55 |
Flow Rate:
12 mL/min |
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Detector
UV @ 254/220 nm (ambient) |
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Samples:
0.2 mL |
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Screw-on Formats
