Better Separation Performance
Tetrandrine Extracted from Natural Products, Formulation
Column A:
Brand X Flash Irregular C18 (40-60 μm
100 Å, 12 g, 3 columns in tandem)
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Column B:
Claricep™ Spherical C18 (20-35 μm 100 Å,
12 g, 3 columns in tandem)
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Column C:
Claricep Spherical C18 (20 μm 100 Å,
12 g, 3 columns in tandem)
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Mobile Phase:
A: Water B: Methanol |
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Gradient:
Time (min)
0
100 | % B
85 85 |
Flow Rate:
83 mL/min |
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Detector:
UV @ 254/282 nm |
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Sample
Tetrandrine |
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Ink Sample Interference / Irregular vs. Spherical
Column A:
Claricep™ Irregular C18 (40-60 μm 60 Å,
20 g, 2 columns in tandem)
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Column B:
Claricep™ Spherical C18 (40-60 μm 100 Å,
20 g, 2 columns in tandem)
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Mobile Phase:
A: Water B: Methanol |
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Gradient:
Time (min)
0
60 | % B
10 100 |
Flow Rate:
26 mL/min |
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Detector:
UV@254 nm (red), @ 220 nm (blue) |
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Instrument:
CHEETAH™ MP 200 |
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Polypeptide Isolation
HPLC Analysis
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Column:
LC C18, 5 μm, 100 Å, 4.6 × 150 mm
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Mobile Phase:
A: Water + 0.01 % TFA
B: Acetonitrile (A/B, 73:27) |
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Flow Rate:
1 mL/min
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Detector:
UV @ 205 nm
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Injection Volume:
1 μL
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Flash Purification
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Column:
Claricep™ C18 (40-60 μm, 100 Å, 12 g)
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Part No.:
SO240012-0
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Mobile Phase:
Acetonitrile/Water
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Flow Rate:
15 mL/min
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Detector:
UV @ 205/280 nm
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Sample Volume:
2 mL
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Purity Test of the Fraction
CLARICEP Spherical C18 Screw-on Columns
Which Screw-on Series should I order ?
CLARICEP i-Series
The i-Series features a managed column head space with a secured screw-on lid. This new design allows either loading of liquid samples directly onto the column head or loading of impregnated solid sample directly into the space. Users will benefit from:
- Choice of loading method based on sample properties
- Narrow band for liquid samples because of wide loading area
- Dry-loading of solid impregnated samples minimizes band broadening
- Customized loading method upon user preference
Ordering Information
Part Number starts with “SN”. As an example, to order SO240012-0 in
i-series, Part Number to order is SN-SO240012-0
CLARICEP c-Series
The c-Series shares the same design, but the column outlet does not have a luer lock structure, this simplifies tubing connection across various flash systems.
Compatible with instruments from the following:- Agela Technologies
- Buchi
- Grace
- Interchim
- Other brands
Ordering Information
Part Number starts with “C”. As an example, to order SO240012-0 in
c-series, Part Number to order is C-SO240012-0
CLARICEP s-Series
The s-Series columns are fully packed without a head void. In addition, the Luer lock fittings for both inlet and outlet allows easy operation of tandem columns or the coupling of a loading cartridge.
Compatible with instruments from the following:- Biotage
Ordering Information
Part Number starts with “S”. As an example, to order SO240012-0 in
s-series, Part Number to order is S-SO240012-0
