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Utilizing Biphasic Salt and pH Gradients for Charge Variant Analysis (TN-1252)
Cation exchange chromatography (CEX) is a favorable analytical method for characterization of proteins as it is non-denaturing and can be used to qualitatively and quantitively identify charge heterogeneity of a protein samples. This technote will describe mobile phase effects on weak cation exchange (WCX) chromatography including a biphasic salt and pH gradient.
Utilizing Biphasic Salt and pH Gradients for Charge Variant Analysis (TN-1252)
Cation exchange chromatography (CEX) is a favorable analytical method for characterization of proteins as it is non-denaturing and can be used to qualitatively and quantitively identify charge heterogeneity of a protein samples. This technote will describe mobile phase effects on weak cation exchange (WCX) chromatography including a biphasic salt and pH gradient.
Document Type:
Technical Notes
Target Industries:
Techniques:
Brands:
Separation Modes:
Ion-Exchange
Phases:
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide