Clarity SPE

The Clarity BioSolutions sample preparation portfolio is composed of unique solid phase extraction chemistries packed in 96-well plate and cartridge formats designed to efficiently and effectively purify oligonucleotides.

Works for desalting and clean up of biological samples
Specially formulated buffer solutions available for optimal performance
Quick protocols for rapid LC-MS/MS results
No liquid-liquid extractions (LLE)

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Oligonucleotide Purification Products

Clarity OTX™ SPE

Clarity OTX™ Extraction Kits

Prior to LC-MS analysis, therapeutic oligonucleotides must be isolated from interferences such as salts, sugars, large proteins, and genomic DNA. Using a mixed-mode solid phase extraction (SPE) sorbent in conjunction with carefully formulated buffers, Clarity OTX consistently delivers high recoveries.

Clarity QSP™ SPE

Clarity QSP™ High-Throughput Purification

Purify trityl-on synthetic DNA or RNA samples in 20 minutes using Clarity QSP 96-well plate and buffer system. Final elution delivers >90 % typical purities/recoveries for in vivo applications and downstream analysis by LC-MS or CE.

Clarity RP-Desalting™

Clarity RP-Desalting™ Quick Salt Removal

Clarity RP-Desalting tubes utilize reversed phase, in lieu of size exclusion, to eliminate salt and reagent from trityl-off synthetic oligo mixtures in order to capitalize on the speed and loading capacity benefits.

Material Characteristics

Clarity Products	Particle Support	Bonded Phase	Particle Shape/Size (μm)	Pore Size(Å)	Surface Area (m²/g)
Clarity OTX™ SPE	Polymer (surface modified PSDVB)	Mixed-mode anion exchanger	33	85	800
Clarity QSP™ SPE	Polymer (PSDVB)	Hydrophilic polymer coating	35	500	300
Clarity RP-Desalting™	Silica	C18	55	140	300

Clarity OTX and Clarity QSP are patented by Phenomenex. U.S. Patent No. 7,119,145

Clarity BioSolutions Product Selection

Purification

	Clarity Oligo-RP™ LC Columns	Clarity Oligo-MS™ LC Columns Clarity Oligo-XT LC Columns	Clarity OTX™ LC Columns
Primary Use	RP-LC-MS analysis with optimized selectivity and sensitivity	Rapid, high efficiency RP-LC-MS analysis for QC and characterization	Extraction of oligo therapeutics from biological samples for LC-MS bioanalysis
Oligo Length	≤ 60 mer	≤ 60 mer	≤ 40 mer
Recommended Mobile Phase	TEA / HFIP	TEA/HFIP/MeOH	N/A

Characterization / Analysis

	Clarity QSP™	Clarity Oligo-RP™ LC Columns Clarity Oligo-XT LC Columns	Clarity RP-Desalting™
Primary Use	High-throughput, trityl-on RPC purification	RP-HPLC purification of failure sequences from target sequences	Quick removal or salt & excess reagent
Purities	>90%	>90%	~70%
Recoveries	~90%	~70%	~70%
Synthesis Scale Load	Up to 50 µmol	Up to 50 µmol	Up to 1 µmol
Oligo Types	DNA, RNA/RNAi, Thioates, Dye-labled, Modified

Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide

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