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Prep Calculator Tool
Preparative HPLC Scale Up Calculator
| Column | Analytical Column | Prep Column |
|---|---|---|
| Particle size (µm) | 5 | 5 |
| Length (mm) | 150 | |
| Diameter (mm) | 4.6 | 10 |
| Column Volume (empty) (mL) | ||
| Equilibration time (min) | ||
| Weight of packing mat (g)* | ||
| Scaling Factor |
| Flow rate (mL/min) | ||
| Sample Amt (mg) | ||
| Injection volume (µL) |
Notes:
- Back pressure stays constant if column length stays constant.
- Back pressure will increase with decrease in particle size.
- Efficiency will increase with decrease in particle size.
- With scale up change in hardware and flow cell may be required.
- * 0.6 g/mL was used. Visit us at Phenomenex.com/Products/Format/Axia
- * Please note all calculations are approximations only. Several factors influence loading capacity of columns. For example:α , solubility, RT, surface area of material, sample type, instrument, etc.
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