Axia Preparative LC Columns
Guaranteed, Longest Lasting Preparative HPLC and SFC Column
Available in over 40 unique achiral and chiral selectivities, Axia advanced preparative column packings and column hardware designs offer several advantages. Unlike traditional preparative HPLC column packing methods, the Axia packing method offers increased sorbent bed density for increased performance and eliminates media bed collapse as a source of premature column failure in preparative HPLC/SFC columns.
Axia Packed Preparative HPLC Columns
Axia Single Axial Compression Step
Axia packed preparative columns involve a single axial compression step, unlike conventional packed preparative columns. The ideal prep HPLC column bed density is custom calculated and automated for each specific media and column size. Computer control of the entire process ensures both proper bed density and column uniformity every time.
Axiaâ„¢ packed columns produce uniform media bed with intact particles
The highly tuned patented process and hardware eliminates potential decompression ensuring bed stability and optimal packing density. The media found on the inlet frit of the Axia packed column shows no signs of damage unlike the media found on inlet frit of traditionally packed HPLC prep columns.
Traditional packed preparative columns produce non-uniform media beds with sheared and crushed particles
Decompression and then recompression during packing can damage the media and lead to increased column-to-column variability, flow disturbances, and decreased column lifetimes.
Unmatched Column Reproducibility
The automated Axiaâ„¢ packing system provides feedback control and infinite tuning of packing density for specific media characteristics such as mechanical strength and porosity. An optimum higher bed density can be consistently reproduced column-to-column. This directly translates into consistent efficiency and peak asymmetry measurements and decreases the column variability seen in traditionally packed preparative columns.
Compare Lifetime
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Columns:
Waters® XTerra® 5 µm Prep MS C18 OBD™
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Dimension:
50 x 21.2 mm (Luna)
50 x 19 mm (XTerra) |
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Mobile Phase:
A: 0.5 % TFA in Water
B: 0.5 % TFA in Acetonitrile Formic Acid |
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Gradient:
Linear 95:5 (A/B) to 5:95 (A/B)
over 7 min, hold 3 min |
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Flow Rate:
30 mL/min (Luna)
24 mL/min (XTerra)‡ |
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Temperature:
Ambient
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Injection Volume:
500 μL
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Detection:
UV @ 254 nm
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Sample:
1. Triprolidine 1.6 mg
2. Methacycline 16 mg 3. Amitriptyline 5.25 mg |
Seamless Scalability to Increase Sample Load
Increase Column Length
- Analytical-like efficiency
- Long column lifetime
- High sample load with high surface area media such as Kinetex, Aeris, Gemini, Luna, Luna Omega, or Synergi
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Gradient:
10 min on 100 mm
length column
25 min on 250 mm
length column
5 % to 95 % Acetonitrile,
0.5 % TFA
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Flow Rate:
30 mL/min
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Increase Column ID
- Reproducible performance across all column diameters
- Increased throughput without sacrificing purity
- High efficiency from analytical to preparative
Analytical 50 x 4.6 mm
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Dimension:
50 x 4.6 mm
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Sample Load:
0.1 mg in 21 µL DMSO
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Flow Rate
1.5 mL/min |
Axia PREP 50 x 21.2 mm
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Dimension:
50 x 21.2 mm
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Sample Load:
2.1 mg in 420 µL DMSO
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Flow Rate
30 mL/min |
Axia PREP 50 x 30 mm
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Dimension:
50 x 30 mm
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Sample Load:
4.2 mg in 840 µL DMSO
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Flow Rate
60 mL/min |
Axia PREP 50 x 50 mm
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Dimension:
50 x 50 mm
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Sample Load:
10.5 mg in 2100 µL DMSO
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Flow Rate
150 mL/min |
Axia Technology Outperforms Traditional Packing Processes!
Warfarin Chiral Purification in Normal Phase Mode
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Column:
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Dimension:
150 x 4.6 mm
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Mobile Phase:
Hexane / Ethanol (75:25)
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Flow Rate:
1 mL/min
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Temperature:
Ambient
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Injection Volume:
100 µL
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Column:
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Dimension:
150 x 21.2 mm
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Mobile Phase:
Hexane / Ethanol (75:25)
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Flow Rate:
20 mL/min
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Temperature:
Ambient
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Injection Volume:
20 mL
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42 % Increase in Axia Efficiency
| Column (mm) |
Analytical 150 x 4.6 |
Standard 150 x 21.2 |
Axia 150 x 21.2 |
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| Mass Loaded (mg) | 2 | 40 | 40 |
| Resolution* | 1.5 | 2.85 | 3.72 |
| Plates (N) | 117 | 535 | 760 |
Axia column and packing technology is patented by Phenomenex. U.S. Patent No. 7, 674, 383
