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mRNA Poly A Tail Degradation Detection on a Biozen Oligo Column
Understanding the degradation of the poly A tail is an essential tool to improving delivery of therapeutics. In this application note, mRNA oligonucleotides strands of 1250 nucleotides were enzymatically digested using RNase H followed by injection into a Biozen 2.6 μm Oligo column. We demonstrate the ability to distinguish a naturally degraded poly A tail with single nucleotide resolution through Liquid Chromatography (LC) and High-Resolution Mass Spectrometry (HRMS).
mRNA Poly A Tail Degradation Detection on a Biozen Oligo Column
Understanding the degradation of the poly A tail is an essential tool to improving delivery of therapeutics. In this application note, mRNA oligonucleotides strands of 1250 nucleotides were enzymatically digested using RNase H followed by injection into a Biozen 2.6 μm Oligo column. We demonstrate the ability to distinguish a naturally degraded poly A tail with single nucleotide resolution through Liquid Chromatography (LC) and High-Resolution Mass Spectrometry (HRMS).
Document Type:
Technical Notes
Target Industries:
Techniques:
Brands:
Separation Modes:
Reversed Phase
Phases:
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mRNA Poly A Tail Degradation Detection on a Biozen Oligo Column
Understanding the degradation of the poly A tail is an essential tool to improving delivery of therapeutics. In this application note, mRNA oligonucleotides strands of 1250 nucleotides were enzymatically digested using RNase H followed by injection into a Biozen 2.6 μm Oligo column. We demonstrate the ability to distinguish a naturally degraded poly A tail with single nucleotide resolution through Liquid Chromatography (LC) and High-Resolution Mass Spectrometry (HRMS).
| Target Industries: | Biopharmaceutical Analysis |
| Techniques: | HPLC |
| Brands: | Biozen LC |
| Separation Modes: | Reversed Phase |
| Phases: | Biozen-Oligo |
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide