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UHPLC/HPLC Method Transfer Tool
Welcome to your UHPLC/HPLC Method Transfer Tool!
As you develop a new method or take on an existing method, it can be time consuming to figure out the exact adjustments needed to transfer said method across different instrumentation, particle morphologies or even LC particle sizes. We've definitely felt that pain over the years and created this Method Transfer Tool to allow you to take back that time!
Enjoy!
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| LC Instrument 1 | Predicted Total Pressure (bar) | LC Instrument 2 | Predicted Total Pressure (bar) | ||||||||
| Pressure Limit (bar) = | 436 | Pressure Limit (bar) = | 397 | ||||||||
| Delay Volume (mL) = | 0.45  | Delay Volume (mL) = | 0.125 | ||||||||
| Instrument Type = | HPLC | Instrument Type = | UHPLC | ||||||||
| Maximum Viscosity = | 1.01E-03 | ACN / H2O 25C | Separation Goal = | Optimum Resolution | |||||||
| LC Column 1 | LC Column 2 | ||||||||
| Length (mm) | Column ID (mm) | Flow Rate (mL/min) | Particle Type / Size (μm) | Porosity | Length (mm) | Column ID (mm) | Flow Rate (mL/min) | Particle Type / Size (μm) | Porosity |
2.6 CS | 0.525 | 50 | 2.1 | 0.45 | 1.7 CS | 0.525 | |||
| Original Gradient Table | Scaled Gradient Table | ||||||||
| Time (min) | %A | %B | #CV | %B/CV | Time | %A | %B | #CV | %B/CV |
| 0 | 1 | 3.30 | 0.00 | 99 | 1 | 1.37 | |||
| 1 | 11.73 | 2.76 | 99 | 1 | 13.66 | ||||
| 13 | 11.73 | 1.02 | 5.13 | 87 | 13 | 11.73 | 1.02 | ||
| 95 | 46.93 | 1.75 | 14.61 | 5 | 95 | 46.93 | 1.75 | ||
| 95 | 5.87 | 15.80 | 5 | 95 | 5.87 | ||||
| 95 | 25.28 | 16.88 | 5 | 95 | 5.37 | ||||
| 1 Column Volume (mL) = | 0.136 | 1 Column Volume (mL) = | 0.091 | ||
| Min Equilibration Time (min) = | 1.244 | Min Equilibration Time (min) = | 1.086 | ||
| Delay Time (min) = | 0.563 | Delay Time (min) = | 0.278 | ||
| Void Time (min) = | 0.170 | Void Time (min) = | 0.202 | ||
| Linear Velocity (mm/s) = | 7.332 | Linear Velocity (mm/s) = | 4.125 | ||
| Max Instrument Delay Volume (mL) = | 1.367 | ||||
| Injection Delay Time (min) = | NaN | ||||
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide