HILIC is a particularly useful separation mode for polar organic compounds. These compounds cannot be retained without HILIC HPLC columns. Retention of these polar compounds using the reversed phase methods is often difficult because of co-elutions with the solvent front or elutions within the chromatographic region where ion suppression is the greatest.
HILIC HPLC/UHPLC columns draw and retain a water-enriched layer onto the surface of the silica, which facilitates the interaction of polar compounds with the stationary phase for increased retention.
Brand | Description: |
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Luna Omega SUGAR | Polyamide/amine phase for the separation of polar analytes such as polyols/sugars |
Kinetex HILIC | Novel core-shell unbonded silica phase provides significant selectivity for retention and separation of polar compounds |
Luna HILIC | Offers excellent MS sensitivity and selectivity for polar compounds |
Luna NH2 | Amine phase applicable to multiple modes of chromatography and classes of polar compounds |
Luna Silica(2) | Ultra-pure, metal-free silica particle that offers great polar selectivity. |
bioZen Glycan | Higher order separations of released N-Glycans and biomarkers. |