5 Reasons Chromatographers Love It
Recommended Use Semi-volatiles, PAHs, and PBDEs, and supreme inertness for acids, amines, and other notoriously active compounds |
Agilent® | Restek® | Supelco® | |
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DB®-5ms DB-5ms Ultra Inert DB-5.625 DB-UI 8270D |
HP-5ms HP-5ms Ultra Inert VP-5ms CP-5il 8 CB MS |
Rxi®-5Sil MS Rxi-5ms |
SLB®-5ms |
Enviro-Inert Technology Improves Inertness Without Changing Selectivity
“Zebron ZB-SemiVolatiles is a very stable and durable semi-volatile column. This has reduced TestAmerica’s downtime and increased our productivity, enabling us to better serve our clients’ needs.”
David Wilkes, GC-MS Semi-Volatile Department
TestAmerica Laboratories, Inc. Buffalo
The opinions stated herein are solely those of the speaker and not necessarily those of any company or organization.
Test Probe / Criteria | Pyridine (PYR) Very active amine – exposes any basic activity |
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EPA 8270D Requirement | Our Requirement | |
Peak Response: | Not Specified | ≥ 0.6* |
Pentachlorophenol (PCP) PCP peaks disappear and exhibit tailing on columns with acid activity |
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EPA 8270D Requirement | Our Requirement | |
Peak Skew: | ≤ 2.0 | ≤ 2.0 |
Peak Response: | Not Specified | ≥ 0.3 |
Benzidine
Active amine that tails when basic activity is present |
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EPA 8270D Requirement | Our Requirement | |
Peak Skew: | ≤ 2.0 | ≤ 2.0 |
DDT Breaks down in an active system to DDE & DDD |
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EPA 8270D Requirement | Our Requirement | |
Breakdown: | < 20 % | < 20 % |
*Requirement of 0.5 for 60 m x 0.25 mm x 0.25 µm and 10 m Guardian™ dimensions
Dimension:
30 meter x 0.25 mm x 0.25 µm
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Injection:
Split 100:1 @ 175 °C, 1 µL
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Carrier Gas:
Hydrogen @ 40 cm/sec (constant pressure)
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Oven Program:
40 °C for 2 min to 300 °C @ 15 °C/min for 3.5 min
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Detector:
FID @ 325 °C |
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Sample:
Analytes are 20 ppm in Dichloromethane 1. Pyridine 2. Pentachlorophenol 3. DFTPP 4. Benzidine 5. DDT 6. DDD |
Pyridine is a very active amine and a good indicator for both column lifetime and sensitivity. Columns with higher initial peak responses can be expected to maintain performance over time. Additionally, higher responses allow you to run at lower levels of detection, improving the sensitivity of your analysis.
Agilent® | PYR | PCP |
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ZB-SemiVolatiles | 1.06 | 0.37 |
Rxi-5ms | 0.34 | 0.34 |
Rxi-5Sil MS | 0.53 | 0.26 |
HP-5ms Ultra Inert | 0.28 | 0.40 |
DB-5ms Ultra Inert | 0.66 | 0.20 |
RF is calculated by dividing peak height of analyte by peak of height of DFTPP as internal standard.
Columns:
Zebron ZB-SemiVolatiles |
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Dimension:
30 meter x 0.25 mm x 0.25 µm |
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Part No.:
7HG-G027-11 |
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Injection:
Split 10:1 @ 280 °C, 1 µL |
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Carrier Gas:
Helium @ 1.4 mL/min (constant flow)
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Oven Program:
40 °C for 0.5 min to 260 °C @ 40 °C/min
to 295 °C @ 6 °C/min to 325 °C @
25 °C/min for 2 min |
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Detector:
MSD @ 340 °C; 45 – 450 amu |
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Liner:
AG0-8499 (Single Taper with Wool) |
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Septum:
AG0-4697 (PhenoRed™ 400) |
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Inlet Seal:
AG0-8620 (Easy Seals™ Inlet Base Seal) |
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Sample:
Analytes are 25 ppm in Dichloromethane135 compounds in EPA Method 8270D |