A large particle, weak anion-exchange functionalized polymeric sorbent that allows for complete retention of acidic compounds with a pKa less than 5, making 100% organic wash conditions possible. A 100% organic wash ensures that the maximum amount of interferences are removed from the target compound. The large particle format makes this sorbent ideal for processing large volumes or viscous samples.
Weak Anion-Exchange
Ï€-Ï€ Bonding
Hydrophobic Interaction
96-Well-Plate
1, 3, and 6mL Tubes
Giga Tubes (12-150mL Tubes)
Column:
1 mL Methanol
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Equilibrate:
1 mL Water, pH 6-7
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Load:
Diluted Sample pH 6-7
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Wash 1:
1 mL 25 mM Ammonium Acetate Buffered, pH 6-7
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Wash 2:
1 mL Methanol
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Elute Any Acids:
2x 500 µL 5 % NH4OH in Methanol
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Elute Weak Acids:
2x 500 µL 5 % Formic Acid in Methanol
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Based on 30 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.