A large particle, strong anion-exchange functionalized polymeric sorbent that allows for complete retention of acidc compounds with a pKa greater than 2, making 100% organic wash conditions possible. A 100% organic wash ensures that the maximum amount of interferences are removed from the target compound. The large particle format makes this sorbent ideal for processing large volumes or viscous samples.
Brand Strata-X |
Recommended Use Extraction of weakly acidic compounds present in viscous samples |
Strong Anion-Exchange
Ï€-Ï€ Bonding
Hydrophobic Interaction
96-Well-Plate
1, 3, and 6mL Tubes
Giga Tubes (12-150mL Tubes)
Column:
1 mL Methanol
|
Equilibrate:
1 mL Water, pH 6-7
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Load:
Diluted Sample pH 6-7
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Wash 1:
1 mL 25 mM Ammonium Acetate Buffered, pH 6-7
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Wash 2:
1 mL Methanol (collect this fraction to analyze Neutral/Bases)
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Elute Acids:
2x 500 µL 5 % Formic Acid in Methanol
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Based on 30 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.