A carboxylic acid group is bonded to the surface of the silica particle, giving a weak cation-exchange selectivity.
96-Well-Plate
1, 3, and 6mL Tubes
Giga Tubes (12-150mL Tubes)
Condition
1 mL Methanol
|
Equilibrate
1 mL DI Water, pH 6-7
|
Load
Pretreated sample, pH 6-7
|
Wash 1
1 mL Water, pH 6-7
|
Wash 2
1 mL Methanol, dry under vacuum for 2-5 min
|
Elute Any Base
1 mL 5% Formic Acid in Methanol
|
Elute Weak Bases
1 mL 5% NH4OH in Methanol
|
Based on 100 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.