Incorporates the hydrophobic selectivity of a C8 phase and strong cation exchange for the extraction of basic drugs from biological matrices.
96-Well-Plate
1, 3, and 6mL Tubes
Condition
1mL Methanol
|
Equilibrate
1 mL Acidified Water
|
Load
Pretreated sample (acidified)
|
Wash 1
1 mL 0.1N HCl in Water
|
Wash 2
1 mL 0.1N HCl in Methanol, dry under vacuum for 2-5 min
|
Elute
1 mL 5 % NH4OH in Methanol
|
Based on 100 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.