The quaternary amine group on this sorbent remains positively charged under all conditions, giving a strong anion-exchange mechanism of retention.
Surface Area (m²/g) m²/g |
Separation Mode Ion Exchange |
Particle Size 55 |
Recommended Use Extraction of weakly acidic compounds |
96-Well-Plate
1, 3, and 6mL Tubes
Giga Tubes (12-150mL Tubes)
Condition
1mL Methanol
|
Equilibrate
1 mL DI Water
|
Load
Pretreated sample, pH 6-7
|
Wash 1
1 mL 25 mM Ammonium Acetate Buffer, pH 6-7
|
Wash 2
1 mL Methanol, dry under vacuum for 2-5 min
|
Elute
1 mL 5 % Formic Acid in Methanol
|
Based on 100 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.