This amino phase offers strong polar selectivity and hydrogen bonding under normal phase conditions or can be used as a weak anion-exchange sorbent.
Surface Area (m²/g) m²/g |
Separation Mode Normal Phase |
Pore Size 100 |
Particle Size 25, 55 |
Recommended Use Strong polar selectivity and hydrogen bonding under normal phase conditions or can be used as a weak anion-exchange sorbent |
96-Well-Plate
1, 3, and 6mL Tubes
Condition
1 mL Methanol
|
Equilibrate
1 mL Water, pH 6-7
|
Load
Pretreated sample, pH 6-7
|
Wash 1
1 mL 25mM Ammonium Acetate Buffer, pH 6-7
|
Wash 2
1 mL Methanol, dry under vacuum for 2-5 min
|
Elute Any Acid
1 mL 5% NH4OH in Methanol
|
Elute Weak Acids
1 mL 5% Formic Acid in Methanol
|
Based on 100 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.