search
Product Information
Phase Detail
Luna PFP(2) HPLC Columns
Luna PFP(2) HPLC columns are packed with a pentafluorophenyl phase that has polar selectivity for cationic compounds
Luna PFP(2) HPLC Columns
Luna PFP(2) HPLC Columns
The Luna brand of columns and media is more than just a product line from Phenomenex. It is a pledge to provide you with the highest level of satisfaction for your chromatographic goals. The Luna PFP(2) is a pentafluorophenyl phase with strongly electronegative fluorine groups to provide polar selectivity for cationic compounds, while the rigid bonded phase is a good steric selector.
- Extremely discerning for halogenated‚ aromatic and conjugated compounds
- Provides orthogonal selectivity even using traditional reversed phase mobile phase systems
- PFP Ultra pure, metal-free silica (99.99 % purity) (USP: L43)/li>
Surface Area (m²/g) 400 |
Particle Size 3, 5 |
Recommended Use Separation of aromatic, halogenated, and closely related compounds |
Long Column Lifetimes and Excellent Performance
Ultra-pure, metal-free silica (99.99 % purity) is the backbone of all Luna material. The resulting high quality particles have a surface smoothness, pore structure, and pore consistency to ensure a more uniform particle shape and greater reproducibility.
Superior Particle Smoothness
Agilent Technologies® ZORBAX® 5 µm SB-C18
Waters® Symmetry® 5 µm C18
Phenomenex Luna 5 µm C18
4 Mechanisms of Interaction
Luna® PFP(2) columns provide unique selectivity for highly polar compounds, complex natural products, isomers and other closely related compounds. This is achieved by using a propyl linked pentafluorophenyl, which provides multiple retention mechanisms unique to typical reversed phase medias.
- Hydrogen Bonding
- Dipole-Dipole Interactions
- Aromatic and π-π Interactions
- Hydrophobic
A typical alkyl phase (C18‚ C8) achieves selectivity through only 1 mechanism of interaction.
Isomeric Compounds
Positional Isomers of Methylacetophenone
Positional changes on an analyte of interest may effect the compound’s dipole moment. This change can be readily seen by the way the highly electronegative fluorine (F) atoms and other retention mechanisms of the Luna PFP(2) are able to separate positional isomers.
The high efficiencies and bonded phase surface coverage provide for sharp peaks The C 8 columns are generally better hydrogen bond acceptors, and better for acidic compounds.
Luna 3 µm PFP(2)
Conditions same for both columns:
|
Column:
Luna 3  µm PFP(2)
Luna 3 µm C18(2) |
|
Dimensions
150 x 4.6  mm
|
|
Part No.:
|
|
Mobile Phase:
Water/Methanol (50:50)
|
|
Flow Rate:
1 mL/min
|
|
Temperature:
22 °C
|
|
Detection:
UV @ 254 nm
|
|
Sample:
1. o-Methylacetophenone
2. m-Methylacetophenone
3. p-Methylacetophenone
|
Aromatic Compounds
Catechins
Aromatic compounds show unique retention characteristics on Luna PFP(2) compared to traditional reversed phase columns. The presence of the aromatic benzene ring in Luna PFP(2) increases the relative attraction between the stationary phase and aromatic analytes, leading to increased retention for these types of compounds.
Luna 3 µm PFP(2)
Conditions same for both columns:
|
Column:
Luna 3  µm PFP(2)
Luna 3 µm C18(2) |
|
Dimensions
150 x 4.6  mm
|
|
Part No.:
|
|
Mobile Phase:
A: 0.1 % Formic acid in Water
B: 0.1  % Formic acid in Acetonitrile
|
|
Gradient:
A/B (80:20) to (55:45) in 10 min
|
|
Flow Rate:
1 mL/min
|
|
Temperature:
22 °C
|
|
Detection:
UV @ 280 nm
|
|
Sample:
1. Gallic acid
2. Epigallo catechin
3. Catechin
4. Epicatechin
5. Epigallocatechin gallate
6. Gallocatechin gallate
7. Epicatechin gallate
8. Catechin gallate
|
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide