Luna C18(2) HPLC Columns
Discover the unmatched performance of the Luna C18(2) column, designed for consistent, high-quality chromatographic results.
Luna is one of the world’s leading LC columns with long lifetimes and excellent performance. The Luna C18(2) phase is an octadecyl silane with ligands bound to the silica surface, resulting in a C18 HPLC column with a very hydrophobic stationary phase that offers great methylene selectivity.
Luna C18(2) columns enable higher flow rates while maintaining excellent resolution and efficiency. The advanced packing technology ensures consistent, high-performance results across both existing and newer HPLC instrumentation.
Features of Luna C18(2) columns:
- Extensive line of rugged particle sizes
- Easy method scalability from HPLC to preparative and bulk purification LC
- C18(2) Ultra-pure, metal-free silica (99.99% purity) (USP:L1)
- Faster separations
- No need for time-consuming method revalidation
- An ideal choice for improving lab productivity and efficiency
Carbon Load 17.5, 17.5 % |
Surface Area (m²/g) 400 |
Particle Size 2.5, 3, 5, 10, 15 |
Recommended Use Recommended for improved methylene selectivity for USP methods or general LC method development |
Long Column Lifetimes and Excellent Performance
Ultra-pure, metal-free silica (99.99 % purity) is the back-bone of all Luna material. The resulting high quality particles have a surface smoothness, pore structure, and pore consistency to ensure a more uniform particle shape and greater reproducibility.
Wide pH Stability
Luna C18(2) columns are renowned for their exceptional pH stability, offering a wide operational range from pH 1.5 to 9.0. This extended pH range is achieved through the high density of the bonded phase. As a result, peak shapes remain sharp and consistent across varying pH levels, ensuring reliable performance. The wide pH stability of Luna C18 columns allows analysts to work above the pKb of basic compounds, rendering them more hydrophobic. This enhances retention, improves peak shape, and boosts reproducibility, making Luna C18(2) an ideal choice for flexible and long-lasting HPLC methods.
Mobile Phase Compatibility
When using HPLC columns, it's crucial to use HPLC-grade solvents and materials while avoiding immiscible solvent/buffer combinations to prevent column damage. To ensure the highest performance, solvent filtration is strongly recommended to eliminate trace impurities from your chosen mobile phase.
All Luna HPLC columns, including the widely used Luna C18(2), are stable in 100% organic conditions, offering flexibility in method development. It is essential to keep the mobile phase pH within the recommended limits for each stationary phase to avoid compromising the column's integrity and lifespan. Adhering to these guidelines ensures optimal column performance, extended longevity, and consistent, reliable results in your chromatographic analyses.
Common Applications on Compounds
Luna C18(2) columns are widely used in various analytical applications due to their versatility and reliability. These columns are particularly effective for the separation and analysis of a broad range of pharmaceutical and bioactive compounds.
Superior Particle Smoothness
Peak Asymmetry Comparison
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Dimension:
150 x 4.6 mm
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Mobile Phase:
Acetonitrile/Water (50:50)
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Flow Rate:
1.0 mL/min
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Temperature:
Ambient
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Detection:
UV @ 254 nm
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Sample:
1. Pyridine
2. Phenol
3. Toluene
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Polar, Acidic Drugs
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Columns:
 Phenomenex Luna 3 µm C18(2) Waters® Symmetry® 3.5 µm C18 |
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Dimension:
75 x 4.6 mm
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Mobile Phase:
20 mM KH2PO4/ Acetonitrile (70:30)
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Flow Rate:
0.75 mL/min
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Detection:
UV @ 202 nm
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Sample:
1. Tolmetin
2. Naproxen
3. Diflunisal
4. Fenoprofen
5. Indomethacin
6. Ibuprofen
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Hydrophobic, Acidic Compounds
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Columns:
Phenomenex Luna® 5 µm C18(2) Thermo Hypersil-Keystone® HyPURITY™ Elite 5 µm C18 |
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Dimension:
150 x 4.6 mm
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Mobile Phase:
20 mM Potassium phosphate, pH 2.5 / Acetonitrile (75:25)
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Flow Rate:
1.5 mL/min
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Temperature:
30 °C
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Detection:
UV @ 254 nm
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Sample:
1. p-Hydroxybenzoic acid
2. Sorbic acid*
3. Benzoic acid*
4. Salicylic acid
5. p-Toluic acid
* Sorbic acid and Benzoic acid co-elute on HyPURITY Elite
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Basic Compounds
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Columns:
Phenomenex Luna® 5 µm C18(2)
Macherey-Nagel® Nucleosil® 5 µm C18 |
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Dimension:
150 x 4.6 mm
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Mobile Phase:
20 mM Potassium phosphate,
pH 2.5/Acetonitrile (75:25)
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Flow Rate:
1.5 mL/min
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Temperature:
30 °C
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Detection:
UV @ 210 nm
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Sample:
1. Maleic acid
2. Triprolidine*
3. Chlorpheniramine*
4. Diphenhydramine*
*Peaks 2-4 adsorb on Nucleosil C18
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