The Kinetex® 2.6 µm Polar C18 is a core-shell based particle with a polar modified surface and a C18 alkyl phase. This versatile column can easily upgrade existing fully porous 5 µm and 3 µm methods to shorten run time, increase sensitivity, and even provide greater resolution with much higher efficiency levels. Combined with being 100 % aqueous stable, the Kinetex Polar C18 is a unique column that combines performance and selectivity.
*Kinetex analytical columns are compatible with all LC system with standard LC column end-fittings threaded in 10-32. Please contact us for any questions and information about fitting compatibility and any other column internal diameters.
Combined C18 and polar modified surface that provides polar and non-polar retention alongside 100 % aqueous stability.
Columns:
Kinetex 2.6 µm Polar C18
AQUA 3 µm C18 |
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Dimension:
150 x 4.6 mm
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Mobile Phase:
A: 20 mM Potassium Phosphate (pH 1.5) B: Methanol |
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Gradient:
Time (min)
0
1 7 7.01 14 | % B
5 30 30 0 0 |
Flow Rate:
1.2 mL/min |
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Temperature:
22 °C |
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Injection Volume:
5 µL |
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Detection:
UV @ 210 nm |
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Analytes:
1. Thiamine 2. Nicotinamide 3. Pyridoxal 4. Pyridoxine 5. Pantothenic Acid |
Columns:
Kinetex 2.6 µm C18
Fully Porous 1.9 µm C18 Kinetex 2.6 µm Polar C18 Kinetex 2.6 µm PS C18 |
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Dimension:
100 x 2.1 mm
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Mobile Phase:
A: Water with 0.1 % Formic Acid B: Acetonitrile with 0.1 % Formic Acid |
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Gradient:
Time (min)
0
4 4.1 7 | % B
3 100 3 3 |
Flow Rate:
0.5 mL/min |
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Temperature:
25 °C |
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Detection:
MS/MS SCIEX® API 4000™ |
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Sample:
Acesulfame |
Column | Back-Pressure (bar) | RT (min) | Peak Height (cps) | Est. T0* (min) |
---|---|---|---|---|
Kinetex 2.6 µm C18 | 252 | 0.67 | 1.8E+06 | 0.34 |
Fully Porous 1.9 µm C18 | 432 | 1.07 | 1.0E+06 | 0.46 |
Kinetex 2.6 µm Polar C18 | 237 | 1.45 | 2.1E+06 | 0.34 |
Kinetex 2.6 µm PS C18 | 241 | 1.85 | 2.2E+06 | 0.34 |