Kinetex Core-Shell C18 HPLC Column
The Go-To Core-Shell Selectivity for Hydrophobic Compounds!
The well-rounded, all-purpose core-shell Kinetex C18 HPLC column produces high efficiencies compared to traditional, fully porous columns. HPLC C18 columns come in various configurations to ensure outstanding performance and are recognized as the most retentive among alkyl-bonded phases. When used with UHPLC systems, the 2.6 µm size achieves similar performance to fully porous sub-2 µm particles but at significantly lower backpressure. Yielding remarkable chromatographic resolution, higher peak capacities, and greater sensitivity, the Kinetex C18 columns maximize the efficiency of every HPLC or UHPLC analysis.
Advantages of Kinetex Core-Shell C18 HPLC Columns
- Compatible with all Analytical HPLC/UHPLC Systems*
- Four fully scalable particle sizes available
- Excellent Reproducibility and Batch-to-Batch Confidence
- USP: L1
*Kinetex C18 analytical columns are compatible with all LC systems with standard LC column end-fittings threaded in 10-32. Please contact us for any questions and information about fitting compatibility and any other column internal diameters.
Compatibility
Balances Retention and Performance
The Kinetex C18 column offers a well-balanced C18 phase that delivers the highest level of hydrophobic selectivity compared to other Kinetex phases.
Upgrade from Traditional Fully Porous to Kinetex Core-Shell
Enhance your analytical method instantly by upgrading to the Kinetex C18 column for superior performance. Take advantage of the fully scalable available particle sizes of Kinetex C18 to bring improved performance to any LC system!
Core-shell columns are best suited for the rapid and precise separation of complex drug mixtures. Improve the quality control process with Kinetex Core-Shell columns.
1.7 µm Fully Porous vs. 1.7 µm Core-Shell
|
Columns:
Kinetex 1.7 µm C18
Traditional 1.7 µm C18 |
|
|
Dimension:
50 x 2.1 mm
|
|
|
Mobile Phase:
Acetonitrile / Water (50:50)
|
|
|
Flow Rate:
0.6 mL/min
|
|
|
Temperature:
25 °C
|
|
|
Detection:
UV @ 254 nm
|
|
|
Instrument:
*Waters® ACQUITY® UPLC®
|
|
|
Sample:
1. Acetophenone
2. Benzene 3. Toluene 4. Naphthalene |
Fully Porous vs. Core-Shell
|
Columns:
Kinetex 2.6 µm C18 100 Å
Traditional 5 µm ODS-3 100 Å |
|
|
Dimension:
Kinetex: 150 x 4.6 mm
Traditional: 250 x 4.6 mm |
|
|
Mobile Phase:
A: 0.1 % Phosphoric acid in Water
B: 0.1 % Phosphoric acid in Acetonitrile |
|
|
Gradient:
5 % to 95 % B in 9 min (150 x 4.6 mm)
5 % to 95 % B in 15 min (250 x 4.6 mm) |
|
|
Flow Rate:
1.8 mL/min
|
|
|
Temperature:
50 °C
|
|
|
Detection:
UV @ 215 nm (22 °C)
|
|
|
Sample:
1. Procainamide
2. Acetaminophen 3. Folic acid 4. Sulfathiazole 5. Acebutolol 6. Dextromethorphan 7. Diphenhydramine 8. Propafenone 9. Amitriptyline 10. Fluoxetine 11. Naproxen 12. Diflunisal 13. Indomethacin |
Performance with Kinetex C18 on HPLC and UHPLC Systems
Fully Porous vs. Core-Shell
Columns:
Kinetex 2.6 µm C18 (Agilent 1100*)
Kinetex 2.6 µm C18 (Agilent 1290) ZORBAX 1.8 µm SB-C18 (Agilent 1290) |
|
Dimension:
100 x 4.6 mm
|
|
Mobile Phase:
A: Water with 0.1% TFA B: Acetonitrile with 0.1% TFA |
|
Gradient:
Time (min)
0
20 | % B
10 70 |
Flow Rate:
1.2 mL/min |
|
Temperature:
Ambient |
|
Detection:
UV @ 210 nm |
|
Sample:
Mupirocin degradants |
|
