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Product Information
Phase Detail
Clarity OTX Pro
Clarity OTX Pro solid phase extraction tubes allow for the isolation of RNA and DNA-based therapeutics
Clarity OTX Pro
Clarity OTX Pro
Standardized Extraction for Complex Therapeutic Oligonucleotides
Clarity OTX Pro is designed to address the inefficiencies in therapeutic oligonucleotide extraction for bioanalysis. With a DNA/RNA-ase free formulation, user-friendly lysis-loading buffer, and a wide range of sorbent bed masses, Clarity OTX Pro enables reliable and reproducible LC/MS analysis of complex oligonucleotides and oligo-conjugates. The inclusion of readily available Proteinase K further simplifies workflows and enhances consistency.
Reliable, Reproducible, and Ready for Method Transfer
- Engineered for complex oligonucleotide therapeutics and novel conjugates
- Day-to-day reproducibility with < 10%
- Well-characterized method development ranges for standardized protocols
Brand Clarity (SPE) |
Recommended Use Extraction of RNA and DNA-based therapeutics and their metabolites from biological matrices. |
Linearity and Accuracy Range of Over 100-fold Dynamic Range
Extracted Standards (Std): Area vs. Concentration 1-900 ng/mL load; R2 = 0.99
| Sample | Actual Concentration (ng/mL) |
Calculated Concentration (ng/mL) |
Accuracy (%) |
|---|---|---|---|
| Std 1 | 0.56 | 0.53 | 93.4 |
| Std 2 | 1.40 | 1.57 | 111.8 |
| Std 3 | 3.51 | 3.87 | 110.3 |
| Std 4 | 8.77 | 9.55 | 108.9 |
| Std 5 | 21.9 | 19.8 | 90.2 |
| Std 6 | 54.8 | 54.1 | 98.7 |
| Std 7 | 137.0 | 131.7 | 96.2 |
| Std 8 | 343.0 | 310.8 | 90.6 |
Full Range TIC 1-900 ng/mL load
High Recovery at Low Concentrations With Minimal Optimization
GalNAc-conjugated siRNA extracted from rat plasma using Clarity OTX Pro.
Extract 1
Post-Extract Spike EIC (2 ng/mL load)
97% Recovery
Extract 2
Post-Extract Spike EIC (2 ng/mL load)
106% Recovery
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide.
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide.