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Product Information
Phase Detail
Biozen N-Glycan Clean-Up<br class="t-last-br" />
Biozen Glycan sample preparation products offer targeted selectivity for N-glycan
Biozen N-Glycan Clean-Up
Biozen N-Glycan Clean-Up
Easy Clean-up of Labeled, Released N-Glycans Using Solid Phase Extraction (SPE)
SPE HILIC stationary phase that excels at retention and recovery labeled released N-glycans. Microelution format allows for streamlined processing and clean-up of small sample volumes.
- Small volume format allows for more concentrated samples
- Minimal elution solvent
- Excellent retention and recovery of labeled, released N-glycans
- Faster clean-up methods to save on sample prep time
Protocol
N-Glycan Clean-up
Reliable recovery and faster results
 STEP 1 |
 STEP 2 |
 STEP 3 |
 STEP 4 |
 STEP 5 |
| Start with Intact Glycoprotein | Deglycosylation Released N-Glycans by PNGase F in buffer solution |
Labeling Glycans 2-AA or 2-AB for accurate and sensitive glycan detection |
Clean-Up Removal of labeling reagent, salts, or surfactants |
Analysis |
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96-Well Plate:
Biozen N-Glycan Clean-up
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Part No.:
8M-S009-NGA
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Condition:
200 µL Water
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Equilibrate:
200 µL Water/Acetonitrile (15:85)
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Load:
Labeled Sample (up to 400 µL of labeled sample)
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Wash:
2x 600 µL Formic acid/Water/Acetonitrile (1:9:90)
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Elute:
3x 30 µL 200 mM Ammonium acetate in Acetonitrile/Water (5:95) of labeled sample)
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Dilute:
100 µL Dimethylformamide and then 210 µL of Acetonitrile
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| Biozen Columns (mm) | Biocompatible Guard Cartridges | |||
| 100 x 2.1 | 150 x 2.1 | for 2.1 mm | holder | |
| Biozen 2.6 μm Glycan |
00D-4773-AN | 00F-4773-AN | AJ0-9800 | AJ0-9000 |
Labeled Glycans from AGP
Sum of Peak Areas for Z-value Calculation
| Glycan | Sum of Peak Area (%) | Multiplication Factor | Charge Number Share |
| Asialo | 0.28 | 0 | 0 |
| Monosialo- | 13.41 | 1 | 13.41 |
| Disialo- | 4.44 | 2 | 8.88 |
| Trisialo- | 52.49 | 3 | 157.47 |
| Tetrasialo- | 29.4 | 4 | 117.6 |
| Total peak area | 100% | Z-value= 297.36 |
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide