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Product Information
Phase Detail
Biozen MagBeads
Biozen Magbeads is a streptavidin coated paramagnetic affinity bead that can be used to isolate proteins and peptides
Biozen MagBeads
Biozen MagBeads
Immunocapture of mAbs Using Magnetic Beads
Biozen MagBeads are used for the purification, clean up, and isolation of proteins and peptide molecules using a paramagnetic affinity bead with a streptavidin coated surface. Magnetic beads offer a rapid solution compared to traditional sample preparation options by maximizing binding capacity with a uniform particle for accurate and reliable results, in less time.
Non-saturated, accurate binding surface orientation
Blocked with hydrophilic copolymer and coupled with biotinylated BSA which:
- Properly orients capture antibody
- Prevents surface crowding and ligand inactivation through non-specific binding
- Enhances dispersion of particles
- Improves binding efficiency
MagBead Protocol
MagBead Activation
Immunocapture
Rituximab Signature Peptide - ASGYTFTSYNMHWVK
Comparison of Dynabeads M-280 vs. Biozen MagBeads
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Column:
Biozen 3 µm Peptide PS-C18
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Dimension:
50 x 2.1 mm
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Part No.:
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Mobile Phase:
A: 0.1 % Formic acid in Water
B: 0.1 % Formic acid in Acetonitrile |
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Gradient:
3-50 % B in 4.5 minutes
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Flow Rate:
0.3 mL/min
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Temperature:
40 °C
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Detection:
SCIEX X500B Q-TOF
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Sample:
Rituximab 1.5 µg/mL (ASGYTFTSYNMHWVK)
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Biozen MagBeads Binding Activity Leads to
Accurate and Sensitive Results
Accurate and Sensitive Results
Rituximab
- Reduction in non-specific binding
- Excellent reproducibility lot-to-lot
| Immunocapture Bead | Correlation Coefficient |
|---|---|
 Thermo® Dynabeads M-280 |
0.9176 |
 Biozen MagBeads, Lot 1 |
0.9914 |
 Biozen MagBeads, Lot 2 |
0.9941 |
Comparative separations may not be representative of all applications.
Troponin
- Increased assay precision
- Improvements in response
| Concentration (ng/mL) |
MyOneâ„¢ Streptavidin T1 % CV Cal Dose | Biozen MagBeads % CV Cal Dose |
|---|---|---|
| 0.3 | 1 | 1.8 |
| 1.2 | 1.8 | 0.7 |
| 5 | 3.6 | 0.4 |
| 25 | 3.2 | 1 |
| 50 | 4.6 | 1.7 |
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide