search
Product Information
Phase Detail
Biozen Size Exclusion Chromatography (dSEC) Columns
Biozen Size Exclusion Chromatography (dSEC) Columns
Biozen Size Exclusion Chromatography (dSEC) Columns
Advanced SEC Silica Particle Technology and Surface Chemistry for Characterizing Biomolecules
The Biozen dSEC columns are packed with low pore volume silica coupled with a proprietary hydrophilic diol-type bonded surface chemistry that prevents the silica surface from interacting with protein samples.
- Exceptionally Robust, Pore Controlled SEC Particle
- Extreme Stability and Exceptional Lifetime
- Reproducible Separations
Separation Mode Gel Filtration Chromatography (GFC) |
Pore Size 200 |
Particle Size 1.8, 3 |
Recommended Use Analysis of monoclonal antibodies, biosimilars and other biomolecules by using specific molecular weights with improved column lifetimes and recovery |
Biozen SEC Proprietary Silica Particle Technology
The Biozen dSEC columns are packed with low pore volume silica coupled with a proprietary hydrophilic diol-type bonded surface chemistry that prevents the silica surface from interacting with protein samples.
Biozen SEC Hydrophilic Surface Chemistry
Improves Aggregate Analysis
Improved Column Lifetime and Performance Stability
Phenomenex’s optimized SEC column loading technology has significantly improved the overall packing density and silica distribution of the columns leading to improved chromatographic lifetime and stability.
Unchanged Performance After 300 Injections
Unchanged Performance After 100 Hours of Extreme Running Conditions
New Standard for Platform SEC Methods
Whether IgG2 or IgG4 isotypes, bispecifics, or Fc-Fusions, dSEC-2 provides excellent separation and sample recovery for many different classes of antibodies and related recombinant proteins.
Conditions for both separations:
|
Column:
Biozen 1.8 µm dSEC-2, 200  Å
|
|
Dimension:
300 x 4.6 mm
|
|
Part No:
00H-4787-E0
|
|
Mobile Phase:
200 Potassium Phosphate +
250 mM KCl, pH 6.2
|
|
Flow Rate:
0.35 mL/min
|
|
Flow Rate:
0.35 mL/min
|
|
Injection Volume:
10 µL
|
|
Temperature:
25 ËšC
|
|
Detection:
UV @ 280 nm
|
|
Sample:
As noted
|
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide