search
Product Information
Phase Detail
Biozen dSEC-1
Achieve high-resolution peptide analysis with Biozen SEC columns. Perfect for separating peptide aggregates and ensuring accurate results.
Biozen dSEC-1
Biozen dSEC-1
High-Resolution Aggregate Analysis for Peptide Therapeutics
Biozen dSEC-1 columns are engineered to meet the growing demand for aggregate analysis in peptide therapeutics and mid-sized biologics. Packed with 90 Ã… small pore silica and featuring inert diol-based surface chemistry, dSEC-1 delivers enhanced resolution for low and high molecular weight peptide aggregates. Its robust design ensures reproducibility and compatibility with advanced workflows like SEC-MS and SEC-MALS.
Robust Performance for Peptide Aggregate Analysis
- High resolution for low/high MW peptide aggregates (1–50 kDa)
- Reproducible, and fast analysis with extended column lifetime
- Improved peak asymmetry by up to 40%
Separation Mode Size Exclusion |
Recommended Use Analysis of peptides, protein fragments, and small biologics with reduced analysis times and improved column lifetimes. |
Aggregate and Monomer Separation in Liraglutide Formulations
- Baseline resolution of monomer and two aggregates achieved in under 10 minutes
- Exceptional reproducibility with retention time RSD ≤ 0.08% and peak area RSD ≤ 0.01%
- Clear detection at low levels with S/N ratios of 13, 75, and 10,917 for aggregates and monomer
LC Conditions
|
Column:
Biozen dSEC-1, 1.6 μm
|
|
Dimension:
150 x 4.6 mm
|
|
Part No:
00F-4801-E0
|
|
Mobile Phase (isocratic):
1X PBS:Isopropanol (70:40 v/v)
|
|
Flow Rate:
0.35 mL/min
|
|
Injection Volume:
20 µg on column
|
|
Temperature:
40 ËšC
|
|
LC System:
Waters H-class
|
|
Detection:
UV@ 280 nm
|
High-Resolution SEC for siRNA Sequence Differentiation
- Clear resolution achieved between closely related species differing by just three bases
- Robust SEC performance enables confident ID and quantification of CholTEG-conjugated siRNA
- Ideal for therapeutic oligo workflows requiring high resolution and reproducibility
| Sequence Name | Bases | Sequence | MW |
|---|---|---|---|
| 19 mer Oligo std | 19 | TTA GGT CCC AAA AGG AGA C | 5845.9 |
| 16 mer Oligo std | 16 | GTG CAT CTT CTG AGG T | 4903.2 |
|
Analytes (Diluent):
ChoITEG conjugated siRNA (Water)
|
|
Column:
Biozen dSEC-1, 3 µm
|
|
Dimension:
300 x 4.6 mm
|
|
Part No.:
00H-4801-E0
|
|
Temperature:
10 ËšC
|
|
Flow Rate:
0.10 mL/min
|
|
Mobile Phase (isocratic):
10 mM Potassium phosphate (pH 7.0), 200 mM Potassium chloride, 40%, Acetonitrile
|
|
Injection Volume:
1 µL
|
|
Instrument:
Thermo Vanquish Horizon
|
|
Wavelength (nm):
UV @ 260 nm
|
Separation of Sense/Antisense Strands in CholTEG-Conjugated siRNA
- Excellent resolution of duplex, sense (5900 Da), and antisense (6900 Da) strands achieved
- Supports formulation and stability studies through clear resolution of structurally related species
| Sequence Name | Bases | Sequence | MW |
|---|---|---|---|
| Sense (S) | 16 | N/A - Chl1TEG | 5900 |
| Antisense (A) | 21 | N/A | 6900 |
|
Analytes (Diluent):
ChoITEG conjugated siRNA (Water)
|
|
Column:
Biozen dSEC-1, 3 µm
|
|
Dimension:
300 x 4.6 mm
|
|
Part No.:
00H-4801-E0
|
|
Temperature:
10 ËšC
|
|
Flow Rate:
0.08 mL/min
|
|
Mobile Phase (isocratic):
10 mM Potassium phosphate (pH 7.0), 200 mM Potassium chloride, 35%, Acetonitrile
|
|
Injection Volume:
1 µL
|
|
Instrument:
Thermo Vanquish Horizon
|
|
Wavelength (nm):
UV @ 260 nm
|
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide.
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide.