Sample Preparation Kits

Sample Preparation kits are an easy way to select and implement a clean up method in your laboratory. Often these kits formats combine multiple clean-up methods, buffers, and a step-by-step implementation method to help start the method. One of the two step kits that Phenomenex supplies is a QuEChERS kit, which typically includes pre-measured packets of salts and solvents to facilitate the extraction and partitioning of analytes from food matrices, followed by a cleanup step to remove unwanted co-extractives. This method is widely used because it simplifies the sample preparation process, making it more efficient and accessible. Offering in a single kit format makes it easier for customers to select what they need based on the method they are trying to run and saves time, additional shipments and part number ordering.

roQ QuEChERS makes it easy to extract multiresidue pesticides from food and environmental samples. It removes unwanted interferences such as organic acids, lipids, pigments, sugars, and more. This kit format has precisely measured and easy-to-pour salt packets for the extraction step in this protocol. The sample is then cleaned up further by dispersive SPE (dSPE). Kits are selected based on the matrix clean-up needs and whether laboratories are extracting following AOAC 2007.01 or EN 15662 guidelines.

Learn more about roQ QuEChERs

Step 1: Extraction

*All roQ Extraction kits contain fifty easy-pour salt packets and fifty 50 mL stand-alone centrifuge tubes.

Step 2: Clean Up/dSPE**

	AOAC 2007.01		EN 15662
	1 mL	8 mL	1 mL	6mL
General	150 mg MgSO4, 50 mg PSA KS0-9511	1200 mg MgSO4, 400 mg PSA KS0-9515	150 mg MgSO4, 25 mg PSA KS0-9503	900 mg MgSO4, 150 mg PSA KS0-9507
Fats and Waxes	150 mg MgSO4, 50 mg PSA  50 mg C18E KS0-9512	1200 mg MgSO4, 400 mg PSA KS0-9515	150 mg MgSO4, 25 mg PSA KS0-9503	900 mg MgSO4, 150 mg PSA KS0-9507
Pigmented	150 mg MgSO4 50 mg PSA 50 mg GCB KS0-9513	1200 mg MgSO4, 400 mg PSA 400 mg GCB KS0-9517	150 mg MgSO4 25 mg PSA 2.5 mg GCB KS0-9505	900 mg MgSO4 150 mg PSA 15 mg GCB KS0-9509
Highly Pigmented	–	–	150 mg MgSO4 25 mg PSA 7.5 mg GCB KS0-9506	900 mg MgSO4 150 mg PSA 45 mg GCB KS0-9510
Pigments and Fats	150 mg MgSO4 50 mg PSA 50 mg GCB 50 mg C18E KS0-9514	1200 mg MgSO4 400 mg PSA 400 mg GCB 400 mg C18E KS0-9518	–	–

**All roQ dSPE kits contain pre-weighed sorbents/salts inside 2 mL or 15 mL centrifuge tubes.

Clarity OTX is another clean-up method for oligonucleotides that can be purchased as a kit, but it is also offered as individual components for ease-of-use. This kit provides mixed-mode solid phase extraction (SPE) sorbent in multiple tube and 96-well plate formats in conjunction with carefully formulated buffers to provide high recoveries and accurate results for LC-MS. Clarity OTX kits allow the flexibility to select a kit format that works for diverse sample sizes and reliable buffers to reduce costly errors that can often occur.

Learn more about Clarity OTX kits

Purification

Characterization / Analysis