EGFP IVT mRNA Aggregation by Size Exclusion Chromatography Using a Biozen™ dSEC-7 Column

This application note showcases the Biozen dSEC-7 LC column's capability in analyzing mRNA aggregates, essential for ensuring the stability and efficacy of mRNA vaccines and therapeutics. The study evaluates the effects of heat treatment, salt concentrations, and flow rates on the separation of mRNA aggregates using size exclusion chromatography (SEC). Key findings indicate that a 100 mM Sodium Chloride concentration provides optimal separation, and lower flow rates enhance resolution. The Biozen dSEC-7 column demonstrates excellent sensitivity and resolution, making it a valuable tool for optimizing mRNA production processes and improving product quality.