Method Transferability and Robustness for Modern Size Exclusion Chromatography Methods

Size exclusion chromatography (SEC) is a non-adsorptive chromatographic modality where large molecules, such as proteins or polymers separate based upon hydrodynamic radius. Larger molecules which cannot permeate all the pores in the chromatographic media are partially excluded, thus elute earlier, wherein smaller molecules which can permeate more pores, elute later. In this technical note, we investigate which method parameters and variables to consider for developing a robust and transferable method.