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Metformin from Human Plasma by Phospholipid Removal and LC-MS/MS (TN-1243)
The fast analysis time demonstrated for the assay of metformin in human plasma would be ideally suited for a bio-equivalence study. Here we have demonstrated an LC-MS/MS assay with a total run time of 2 minutes using a Kinetex C18 5 µm, 50 x 4.6 mm column. Sample preparation with the Phree 96-well plate is an automation friendly format, which gave very clean extraction results by minimizing the possible matrix interference peaks from endogenous phospholipids and proteins.
Metformin from Human Plasma by Phospholipid Removal and LC-MS/MS (TN-1243)
The fast analysis time demonstrated for the assay of metformin in human plasma would be ideally suited for a bio-equivalence study. Here we have demonstrated an LC-MS/MS assay with a total run time of 2 minutes using a Kinetex C18 5 µm, 50 x 4.6 mm column. Sample preparation with the Phree 96-well plate is an automation friendly format, which gave very clean extraction results by minimizing the possible matrix interference peaks from endogenous phospholipids and proteins.
Document Type:
Technical Notes
Target Industries:
Separation Modes:
Reversed Phase
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide