Chromatographic Considerations for LC-MS/MS Analysis of Amphetamine in the Presence of Gabapentin using Kinetex Core-Shell LC Columns (TN-1247)

Since gabapentin is unmetabolized and excreted renally, it is found in high concentrations in urine. These high concentrations can make analysis of other compounds, specifically amphetamine, difficult due to overloading the LC column and saturating the source of the mass spectrometer. This work examines the most commonly used drug research panel method conditions and evaluate modifications that can be made to alleviate these issues for robust analysis of amphetamine in a high concentration gabapentin positive sample.