Development of a Two Step Liraglutide Purification Process on a Single Stationary Phase (TN-1250)

A 2-step process was successfully developed for the purification of Liraglutide. Both steps used Luna 10µm PREP C8(3) as the stationary phase. The crude Liraglutide sample for this study had an initial purity of 30%. The first step upgraded the purity to 91%. A second polishing step was needed and elevated the purity to the desired 98.5%. During the development process, the use of a single stationary phase was a primary objective to minimize the overall cost of the methodology.