Improving Resolution for Size Exclusion Chromatography Methods by Optimization of Linear Velocity

Flow rate is a common method parameter for most adsorptive LC methods. However, SEC is a unique separation modality in that lower flow rates often yield improvements in separation and resolution can improve quite considerably simply by running at a lower linear velocity. This application note demonstrates the utility of method improvements by running at a lower flow rate using the Biozen dSEC-2 SEC column for the aggregate analysis of NIST mAb, a common standard protein.