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Hydrocortisone Standard Unfiltered with Guard
26460
Separation Mode: Reversed Phase
Reversed Phase
C18(2)
HPLC
Pharmaceutical/Biopharmaceutical
Pharmaceutical
Therapeutic / Clinical
Analytes
Details
LC Conditions (App ID: 26460)
Column: |
Brand Name: Luna |
Part No: |
Phase Name: C18(2) |
System: Agilent Technologies 1290 Infinity Agilent Technologies 1290 Infinity |
Sample Note: Hydrocortisone cream 1. Dissolve 1g of Hydrocortisone cream in 10mL ethyl acetate (n=6)
2. Dilute 2mL of ethyl acetate-cream sample with 8mL methanol:water (1:1)
3. Load 400uL methanol:water (1:1) cream sample
4. Push sample through filter vial
5. Analyze final eluate via HPLC-UV |
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Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide