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Applications
Extraction of 5-Fluorouracil from Human Serum Using Strata-X PRO SPE on a Kinetex 2.6 μm PS C18 (ESI -Ve Mode)
25778
Separation Mode: Reversed Phase
Reversed Phase
PS C18
HPLC
Clinical
Clinical Research
Therapeutic / Clinical

Extraction of 5-Fluorouracil from Human Serum Using Strata-X PRO SPE on a Kinetex 2.6 μm PS C18 (ESI -Ve Mode)

Analytes
15FU
25 Cl Uracil (-Ve IS)
Details
LC Conditions (App ID: 25778)
Column:
Brand Name:
Kinetex
Part No:
Phase Name:
PS C18
System:
Agilent Technologies 1260 Infinity Agilent Technologies 1260 Infinity
Sample Note:
Add 100 µL Serum (doubly stripped) to tube. Add 900 µL of chilled (~00C) Acetonitrile to the serum while vortexing. Centrifuge samples at 6000 rpm for 5 minutes and transfer supernatant. Analyte diluted with initial mobile phase.
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