| 1C12:0 |
|---|
| 2C14:0 |
| 3C16:0 |
| 4C16:1 cis 9 |
| 5C18:0 |
| 6C18:1 cis 9 |
| 7C18:2 cis 9,12 |
| 8C18:3 cis 9,12,15 |
| 9C20:0 |
| 10C20:1 cis 11 |
search
Applications
FAMEs In Palm Oil by GC/FID on Zebron ZB-FAME
23969
Separation Mode: Application Specific
Application Specific
ZB-FAME
GC
Enzyme Inhibitors
Food & Beverage
Analytes
Details
GC Conditions (App ID: 23969)
Column: |
Part No: |
Phase Name: ZB-FAME |
Sample Notes: Recommended Accessories:
Recommended Liner: Zebron PLUS Single Taper with Wool, 4 mm ID
Liner Part No.: AG2-0A11-05 (for Agilent systems)
Inlet Seal: AG0-8620 (Gold Plated Easy Seal)
Septum: AG0-4696 (PhenoRed-400)
Sample Preparation:
1. Weigh 0.2 g of sample into a glass test tube
2. Add 3.8 mL of hexane and vortex
3. Condition SPE tube (Strata® Si-1, 2 g/12 mL, Part No.: 8B-S012-KDG) under vacuum with 6 mL of hexane; do not let bed go dry
4. Load sample with light vacuum
5. Elute with 5 mL of hexane:ethyl acetate (87:13)
6. Evaporate under nitrogen at 35 °C
7. Reconstitute with 4 mL of hexane
8. Add 200 µL of 2 M potassium hydroxide/methanol solution and vortex
9. Leave at room temperature for 5 minutes
10. Add 2 mL of deionized water and vortex
11. Leave to separate until upper layer becomes clear
12. Extract upper layer for GC analysis |
Similar Applications
search
No Data
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide