| 1Triphenylene (I.S.) |
|---|
| 2Vitamin D3 |
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Applications
Vitamin D tablet assay (Naturemade 2000 IU) on a Kinetex 2.6 µm Biphenyl, 150 x 4.6
23496
Separation Mode: Reversed Phase
Reversed Phase
Biphenyl
HPLC
Vitamins
Analytes
Details
LC Conditions (App ID: 23496)
Column: |
Brand Name: Kinetex |
Part No: |
Phase Name: Biphenyl |
Sample Note: Sample preparation: small tablets (<400 mg)
1. Grind tablet into powder and transfer to a 2 mL dSPE tube (KS0-8916)
2. Spike in 20 µL of triphenylene internal standard (1 mg/mL) and standard additions (if applicable)
3. Add 800 µL of water
4. Sonicate until dissolved (approx. 5 min)
5. Add 800 µL of acetonitrile
6. Shake for 10 min using mechanical shaker
7. Centrifuge at 15000 rpm for 3 min
8. Aspirate 100 µL of supernatant and transfer to an autosampler vial for analysis
Sample preparation: large tablets (>400 mg)
1. Grind tablet into powder and transfer to a 5 mL centrifuge tube
2. Spike in 20 µL of triphenylene internal standard (1 mg/mL) and standard additions (if applicable)
3. Add 2 mL of water
4. Sonicate until dissolved (approx. 5 min)
5. Add 400 mg of Quechers salt (AH0-9044)
6. Add 2 mL of acetonitrile
7. Shake for 10 min using mechanical shaker
8. Centrifuge at 6000 rpm for 3 min
9. Aspirate 100 µL of supernatant and transfer to an autosampler vial for analysis |
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Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide