| 1Timolol |
|---|
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Applications
Extraction of Timolol from Serum using Strata-X-C 10 mg Plate & Kinetex 2.6u Biphenyl 50x2.1 Column
22982
Separation Mode: Reversed Phase
Reversed Phase
Biphenyl
HPLC
Antihypertensive Agents
Extraction of Timolol from Serum using Strata-X-C 10 mg Plate & Kinetex 2.6u Biphenyl 50x2.1 Column
Analytes
Details
LC Conditions (App ID: 22982)
Column: |
Brand Name: Kinetex |
Part No: |
Phase Name: Biphenyl |
Sample Note: Strata X-C 10 mg Plate Protocol
Condition
500 µL methanol
Equilibrate
500uL water
Load
750 µL diluted serum
(375 µL plasma diluted 1:1 with 4% phosphoric acid in water)
Wash1
500 µL 2% formic acid in water
Wash 2
500 µL methanol
Elute
375 µL(3x125 µL) 5% ammonium hydroxide in acetonitrile:methanol(60:40)
Inject
1 µL
Note: Spiked conc. of analyte was 1 ng/mL serum |
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Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide