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Applications
Blank Baby Wash using Novum SLE 12cc Tube & Kinetex 5u EVO C18, 100x3.0
22924
Separation Mode: Reversed Phase
Reversed Phase
EVO C18
HPLC
Personal Care/Consumer Products
Personal Healthcare / Cosmetic
Product / Trade Good
Analytes
Details
LC Conditions (App ID: 22924)
Column: |
Brand Name: Kinetex |
Part No: |
Phase Name: EVO C18 |
Sample Note: Novum SLE 12cc Tube
8B-S138-KDG
Novum SLE 12cc Tube
Extraction Procedure
Sample pre-treatment
0.5 ± 0.05 g spiked Aveeno Baby (Wash & Shampoo) sample
Add 1.25 mL acetone
Add 0.6 ml 1% Nacl solution
Vortex for 30 secs
Wait 30 secs till the emulsion dissipates
Centrifuge for 10 mins at 3000 rpm
Collect the supernatant and proceed to load
Sample loading
"Load the sample from pre-treatment step above onto the Novum SLE cartridge and apply a short and gentle pulse of vacuum (~ 5" of Hg for 5-10 sec or until the sample has completely entered the media).
"Wait for 5 minutes.
Elution
"Dispense 10 mL of ethylacetate onto the SLE media and allow the solvent to elute by gravity for 10 mins and collect the eluant.
"Apply vacuum at 5"-10"of Hg to complete the extraction.
NOTE: Prolonged application of vacuum will result in elution of body wash out of the SLE media and into the final extracted solvent.
Dry down
"Evaporate the final extract to complete dryness under slow stream of N2 at 45°C
Reconstitute
"Reconstitute the dry residue in 0.5 mL of methanol
"Centrifuge for 5 mins at 6000 rpm
"Collect supernatant to shoot on HPLC |
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Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide