| 125 Hydroxy vitamin D3-2H3 |
|---|
| 225 Hydroxy vitamin D3 |
| 325 Hydroxy vitamin D2 |
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Applications
Extraction of Mono Hydroxy Vitamin D2 and D3 from Phree on Kinetex C18 2.6u 30x3 mm
21868
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Vitamins
Clinical
Analytes
Details
LC Conditions (App ID: 21868)
Column: |
Brand Name: Kinetex |
Part No: |
Phase Name: C18 |
Sample Note: Instrument: API 5000 (AB SCIEX)
Ionization Source: APCI, Positive Polarity
Ionization Source Parameters
Curtain Gas: 25.0 psi
Gas 1 (Neb Gas): 40.00 psi
ihe: ON
CAD (Collision Gas): 10.0
NC: 5.00 µA
TEM: 360 °C
Note: Gas 2 is not available in APCI source
MRM Transitions and Mass Dependent Parameters:
Compound Q1, Da Q3, Da Dwell, msec DP, V EP, V CE, V CXP, V
Vit D2 (1) 395.3 209.3 45 69 10 25 10
Vit D2 (2) 395.3 269.2 45 69 10 25 10
IS (D3-3H2) 386.2 257.2 45 69 10 25 10
Vit D3 (1) 383.2 257.2 45 69 10 23 10
Vit D3 (2) 383.2 211.1 45 69 10 34 10 |
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Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide