Product Information
Brand
Strata solid phase extraction (SPE) products provide reproducible recoveries and cleanup. Strata SPE sorbents are available in the following formats SPE Tubes, 96-well plates, online SPE, and Giga tubes
Strata SPE products are manufactured to bring you optimal quality, reproducibility, and performance. With over 20 years of experience, Strata SPE products provide clean extracts and high recoveries for a multitude of target analytes across diverse sample matrices.
- Available retention mechanisms include reversed phase, ion exchange, normal phase and mixed mode
- 24 phases and counting
- Cleaner extracts and reproducible results
Available Formats
96-Well-Plate
1, 3, and 6mL Tubes
Giga Tubes (12-150mL Tubes)
Identify the Specific SPE Retention Mechanism
| SPE Mechanism |
Analyte Functional Group |
Sorbent Functional Group |
Strata Sorbent |
|---|---|---|---|
| Reversed Phase |
R   hydrocarbon 
 aromatic |
R   hydrocarbon 
 aromatic |
C18-E, C18-U, C8 C18-T PH, SDBL |
| Ion-Exchange |
NR4+ strong RNH3+ weak RSO3- strong RCO2- weak |
-O2C—weak -O3S—strong +H3N—weak +R3N—strong |
WCX Screen-C, SCX NH2 Screen-A, SAX |
Silica-Based Sorbents Loading Capacities
| Sample Matrix | Sorbent Mass |
|---|---|
| Blood, serum, plasma | 50 mg sorbent per 250 µL |
| Urine | 50 mg sorbent per 500 µL |
| Filtered tissue homogenates | 100 mg sorbent per 100 mg tissue |
| Environmental Samples | Sorbent Mass |
|---|---|
| Water (particulate-free) drinking | 500 mg/100 mL - 500 mL sample |
| Water (particulate-laden) rivers, runoff, etc. | 1 g/100 mL - 500 mL sample |
| Soil extracts | 1 g/100 g of soil extract |
| Food/Plant Material | 1 g/ 100g sample |
Sorbent Wash and Elution Volumes*
The volume of solvent needed for the wash and elution steps is directly related to the mass of sorbent in the SPE tube and more specifically the “bed volume” of the SPE device. Typically 4 – 16 bed volumes are used in SPE methods.
| Sorbent Mass | 10 mg | 50 mg | 100 mg | 150 mg | 200 mg | 500 mg | 1 g | 2 g | 5 g | 10 g |
|---|---|---|---|---|---|---|---|---|---|---|
| Practical Minimum Wash and Elution Volume 4 bed volumes |
60 µL | 300 µL | 600 µL | 900 µL | 1.2 mL | 3 mL | 6 mL | 12 mL | 30 mL | 60 mL |
| Recommended Wash and Elution Volume 8 bed volumes |
120 µL | 600 µL | 1.2 mL | 1.8 mL | 2.4 mL | 6 mL | 12 mL | 24 mL | 60 mL | 120 mL |
General Starting Method Reversed Phase
Column:
1 mL Methanol |
Equilibrate:
1 mL DI Water |
Load:
Pretreated sample |
Wash:
1 mL 5% Methanol in DI Water, dry under
vacuum for 2-5 min |
Elute:
1 mL Methanol |
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