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Product Information
Phase Detail
Strata-X-Drug B and Strata-X-Drug B Plus SPE Products
Strata-X-Drug-B solid phase extraction (SPE) sorbent is a polymeric specialty phase that can be used to cleanup basic drugs of abuse
Strata-X-Drug B and Strata-X-Drug B Plus SPE Products
Strata-X-Drug B and Strata-X-Drug B Plus SPE Products
Quick and In-Well Hydrolysis Solid Phase Extraction (SPE)
Proprietary sorbent that is designed and quality controlled for basic drugs of abuse analysis. This sorbent does not require a conditioning/equilibration step to save time and help improve throughput. With Strata-X-Drug B Plus, the hydrolysis step is performed in the well, providing even more time savings and reducing transfer steps.
- QC Tested for Drug of Abuse
- No Conditioning or Equilibration Steps Required
Brand Strata-X |
Recommended Use Extraction of basic drugs from biological samples |
General Extraction
- Extract over 11 drugs using the same sorbent
- Consolidate methods; 3 methods is all you’ll need to extract 6 different drug classes
- Time and solvent savings; no conditioning required
- Consistent, sensitive, and high recoveries of SAMHSA regulated drugs
Retention Mechanism
Strata-X Drug B Protocol
Strata-X Drug B Plus Protocol
Starting Method
General Urine Pre-treatment Recommendation
- Combine urine sample with appropriate buffer.
- Add β-Glucuronidase enzyme according to manufacturer’s recommendation (If using Strata-X-Drug B Plus, these steps can be performed in-well).
- Incubate for specified amount of time (If using Strata-X-Drug B Plus, pulse vacuum to load solution onto sorbent).
- Follow appropriate SPE protocol based on analytes of interest.
Choose Your Analytes
| Opiates, 6-MAM, PCP, Amphetamines, Propoxyphene, Healthcare Opiates, and Methadone* |
Marijuana Metabolites | Cocaine Metabolites | |
|---|---|---|---|
| Condition | Not Required | Not Required | Not Required |
| Load | Pre-treated urine sample | Pre-treated urine sample | Pre-treated urine sample |
| Wash 1 | 600 µL of 100 mM Sodium acetate buffer (pH 5.0) | 600 µL of 100 mM Sodium acetate buffer (pH 5.0) | 600 µL of 0.1 N Hydrochloric acid |
| Wash 2 | 600 µL Methanol | 600 µL of Acetonitrile/100 mM Sodium acetate buffer (pH 5.0) (30:70) | 600 µL Methanol |
| Dry | 10 minutes under full vacuum | 15 minutes under full vacuum | 10 minutes under full vacuum |
| Elute | 2x 300 µL of Ethyl acetate/Isopropanol/ Ammonium hydroxide (70:20:10) |
2x 300 µL of Ethyl acetate/Isopropanol (85:15) |
2x 300 µL of Ethyl acetate/Isopropanol/ Ammonium hydroxide (70:20:10) |
* Opiates, 6-MAM, PCP, Amphetamines, Propoxyphene, Healthcare Opiates, and Methadone can be extracted simultaneously or separately using the same SPE methodology. Methods are written for 30 mg/well Strata-X-Drug B 96-well plate; however they can be scaled to accommodate smaller or larger sample sizes and sorbent masses.
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide