Applications

Cytochrome C Tryptic Digest Map

Analytes

1Cytochrome c

Compound Name Myohematin
CID 439171
Molecular Formula C42H52FeN8O6S2
Molecular Weight 884
No. Chiral Centers: 4
No. Hydrogen Bond Acceptors 12
No. Hydrogen Bond Donors 6

Details

LC Conditions (App ID: 8716)

Column:

Jupiter 5 µm C18 300 Å, LC Column 250 x 4.6 mm, Ea

Brand Name:

Jupiter

Part No:

00G-4053-E0

Phase Name:

C18

Elution Type:

Gradient

Mobile Phase:

A: 0.1% TFA in water

B: 0.08% TFA in acetonitrile

Gradient Profile:

Step No.	Time(min)	%A	%B
1	0	95	5
2	30	35	65

Flow Rate:

1 mL/min

Temperature:

22°C

Detection:

UV-Vis Abs.-Variable Wave.(UV) @ 215 nm (22°C)

Sample Note:

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, Widepore C18 4 x 3.0mm, 10/Pk Part No.: AJ0-4321 - Holder Part No.: KJ0-4282

Order Items Used in This Application

00G-4053-E0

Jupiter 5 µm C18 300 Å, LC Column 250 x 4.6 mm, Ea

Qty:

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