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Applications
Trimethoprim and Sulfamethoxazole
5645
Separation Mode: Reversed Phase
Reversed Phase
ODS (30)
HPLC
Anti-Infective Agents
Pharmaceutical
Therapeutic / Clinical

Applications

Trimethoprim and Sulfamethoxazole
Analytes
1Trimethoprim
2Sulfamethoxazole
Compound Name
Trimethoprim
CID
5578
Molecular Formula
C14H18N4O3
Molecular Weight
290
No. Hydrogen Bond Acceptors
7
No. Hydrogen Bond Donors
2
Details
LC Conditions (App ID: 5645)
Column:
Brand Name:
Part No:
Phase Name:
ODS (30)
Elution Type:
Isocratic
Mobile Phase:
A: Water
B: Acetonitrile containing 0.1% TEA, pH 5.9 with acetic acid
Gradient Profile:
Step No.Time(min)%A%B
108020
Flow Rate:
2 mL/min
Temperature:
22°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) @ 254 nm (22°C)
Sample Note:
SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, C18 4 x 3.0mm, 10/Pk Part No.: AJ0-4287 - Holder Part No.: KJ0-4282

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