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Applications
Prednisolone - USP Acceptable Modified Method
3269
Separation Mode: Reversed Phase
Reversed Phase
USP L03
C8(2)
HPLC
Antineoplastic Agents
Pharmaceutical
Therapeutic / Clinical

Applications

Prednisolone - USP Acceptable Modified Method
Analytes
1Betamethasone
2Prednisolone
Compound Name
Betamethasone
CID
9782
Molecular Formula
C22H29FO5
Molecular Weight
392
No. Hydrogen Bond Acceptors
6
No. Hydrogen Bond Donors
3
Details
Official Method: USP L03
LC Conditions (App ID: 3269)
Column:
Brand Name:
Part No:
Phase Name:
C8(2)
Elution Type:
Isocratic
Mobile Phase:
A: Butyl chloride:water-saturated butyl chloride:tetrahydrofuran:methanol:glacial acetic acid (95:95:14:7:6)
Gradient Profile:
Step No.Time(min)%A
10100
Flow Rate:
1.5 mL/min
Temperature:
22°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) @ 254 nm (22°C)
Sample Note:
SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, C8 4 x 3.0mm, 10/Pk Part No.: AJ0-4290 - Holder Part No.: KJ0-4282

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