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Applications
Proteins
2562
Separation Mode: Reversed Phase
Reversed Phase
C8(2)
HPLC
Hypoglycemic Agents
Protein
Biochemical Compound / Nutrient

Applications

Proteins
Analytes
1Insulin
2Insulin intermediate
3Insulin intermediate
4Proinsulin

Analyte not found

Details
LC Conditions (App ID: 2562)
Column:
Brand Name:
Part No:
Phase Name:
C8(2)
Elution Type:
Gradient
Mobile Phase:
A: 0.085% Phosphoric acid, 0.3% TEA, pH 7.0 with NaOH
B: Solution A/Acetonitrile (40/60)
Gradient Profile:
Step No.Time(min)%A%B
105545
2303268
Flow Rate:
1.5 mL/min
Temperature:
22°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) @ 210 nm (22°C)
Sample Note:
SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, C8 4 x 3.0mm, 10/Pk Part No.: AJ0-4290 - Holder Part No.: KJ0-4282

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